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Literature summary for 2.5.1.61 extracted from

  • Li, N.; Chu, X.; Wu, L.; Liu, X.; Li, D.
    Functional studies of rat hydroxymethylbilane synthase (2008), Bioorg. Chem., 36, 241-251.
    View publication on PubMed
Search for more literature for 2.5.1.61

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21(DE3) Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
D44A specific activity is only about 2% of that of the wild type enzyme Rattus norvegicus
E63A reduced activity Rattus norvegicus
H78L reduced activity Rattus norvegicus
Q200L mutant lacks the dipyrromethane cofactor and completely loses the catalytic activity Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
 porphobilinogen wild type enzyme, at 37°C in Tris-HCl buffer (100 mM, pH 8.0, containing 0.15 mg/ml BSA) Rattus norvegicus
0.066
-
 porphobilinogen mutant enzyme E63A, at 37°C in Tris-HCl buffer (100 mM, pH 8.0, containing 0.15 mg/ml BSA) Rattus norvegicus
0.24
-
 porphobilinogen mutant enzyme E63A, at 37°C in Tris-HCl buffer (100 mM, pH 8.0, containing 0.15 mg/ml BSA) Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus P19356
-
-

Purification (Commentary)

Purification (Comment) Organism
Hi-Trap chelating metal affinity column chromatography Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.001
-
 mutant enzyme D44A, at 37°C Rattus norvegicus

Storage Stability

Storage Stability Organism
-80°C, 50 mM Tris buffer, pH 7.5, 5% glycerol, and 5 mM beta-mercaptoethanol, 1 year, remains stable Rattus norvegicus
4°C, 50 mM Tris buffer, pH 7.5, 5% glycerol, and 5 mM beta-mercaptoethanol, 1 week, without significant change of activity Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4 porphobilinogen + H2O
-
 Rattus norvegicus hydroxylmethylbilane + 4 NH3
-
 ?

Synonyms

Synonyms Comment Organism
EC 4.3.1.8 formerly Rattus norvegicus
HMB synthase
-
 Rattus norvegicus
PBG deaminase
-
 Rattus norvegicus
porphobilinogen deaminase
-
 Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 only a little loss of activity is found even after 1 h preincubation at 60°C Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
6 9
-
 Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
dipyrromethane
-
 Rattus norvegicus