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Literature summary for 2.5.1.61 extracted from
- Purification and properties of porphobilinogen deaminase from Arabidopsis thaliana (1994), Biochem. J., 299, 895-902.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-bromoporphobilinogen | - |
Arabidopsis thaliana |  |
| Ag2+ | 1 mM AgCl2, 90% inhibition | Arabidopsis thaliana | |
| Cd2+ | 0.1 mM CdCl2, 55% inhibition | Arabidopsis thaliana | |
| Co2+ | 0.1 mM CoCl2, 95% inhibition | Arabidopsis thaliana | |
| Cu2+ | 1 mM CuSO4, 66% inhibition | Arabidopsis thaliana | |
| Hg2+ | 0.1 mm HgCl2, 76% inhibition | Arabidopsis thaliana | |
| hydroxylamine | - |
Arabidopsis thaliana | |
| K+ | at high concentrations | Arabidopsis thaliana | |
| additional information | - |
Arabidopsis thaliana | |
| Na+ | at high concentrations | Arabidopsis thaliana | |
| NH4+ | - |
Arabidopsis thaliana | |
| Zn2+ | 1 mM ZnCl2, 78% inhibition | Arabidopsis thaliana | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.017 | - |
porphobilinogen | - |
Arabidopsis thaliana | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
gel filtration | Arabidopsis thaliana |
| 35000 | - |
1 * 35000, SDS-PAGE | Arabidopsis thaliana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| porphobilinogen | Arabidopsis thaliana | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
Columbia | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Arabidopsis thaliana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Arabidopsis thaliana | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Arabidopsis thaliana |
| 0.073 | - |
- |
Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4 porphobilinogen | in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 | Arabidopsis thaliana | uroporphyrinogen III + 4 NH3 | - |
? | |
| 4 porphobilinogen + H2O | - |
Arabidopsis thaliana | hydroxylmethylbilane + 4 NH3 | 1-hydroxymethylbilane, highly unstable | ? | |
| porphobilinogen | - |
Arabidopsis thaliana | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 35000, SDS-PAGE | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| porphobilinogen deaminase | - |
Arabidopsis thaliana |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
10 min, heat-stable up to 70°C | Arabidopsis thaliana |
| 80 | - |
10 min, 40% loss of activity | Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.7 | 8.5 | - |
Arabidopsis thaliana |
| 8 | - |
- |
Arabidopsis thaliana |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | pH 6.0: about 15% of maximal activity, pH 10.0: 25% of maximal activity | Arabidopsis thaliana |
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