Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | CaCl2, weak | Pisum sativum | |
Co2+ | CoCl2, strong | Pisum sativum | |
Fe2+ | FeSO4, strong | Pisum sativum | |
Mg2+ | MgCl2, weak | Pisum sativum | |
Mn2+ | MnCl2; strong inhibition at submillimolar concentrations | Pisum sativum | |
Na+ | NaCl greater than 300 mM | Pisum sativum | |
NH4+ | - |
Pisum sativum | |
Zn2+ | ZnCl2, strong | Pisum sativum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Pisum sativum | 9507 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
1 * 45000, SDS-PAGE | Pisum sativum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
porphobilinogen | Pisum sativum | - |
? | - |
? | |
porphobilinogen | Glycine max | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
soya-bean | - |
Pisum sativum | - |
pea | - |
Purification (Comment) | Organism |
---|---|
dye-ligand affinity chromatography | Pisum sativum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
callus | - |
Glycine max | - |
leaf | - |
Pisum sativum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0813 | - |
- |
Pisum sativum |
Storage Stability | Organism |
---|---|
-20°C or 4°C, unstable, loss of activity after several days | Pisum sativum |
-20°C, crude plastide lysate is stable for at least 2 weeks | Pisum sativum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4 porphobilinogen | in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 | Pisum sativum | uroporphyrinogen III + 4 NH3 | - |
? | |
4 porphobilinogen | in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 | Glycine max | uroporphyrinogen III + 4 NH3 | - |
? | |
4 porphobilinogen + H2O | - |
Pisum sativum | hydroxylmethylbilane + 4 NH3 | - |
? | |
4 porphobilinogen + H2O | - |
Glycine max | hydroxylmethylbilane + 4 NH3 | - |
? | |
porphobilinogen | - |
Pisum sativum | ? | - |
? | |
porphobilinogen | - |
Glycine max | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 45000, SDS-PAGE | Pisum sativum |
Synonyms | Comment | Organism |
---|---|---|
porphobilinogen deaminase | - |
Pisum sativum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | 8.2 | - |
Pisum sativum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | pH 6.5: 25% of maximal activity, pH 9.0: 50% of maximal activity | Pisum sativum |