Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.61 extracted from

  • Davies, R.C.; Neuberger, A.
    Polypyrroles formed from porphobilinogen and amines by uroporphyrinogen synthetase of Rhodopseudomonas spheroides (1973), Biochem. J., 133, 471-492.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.61

Inhibitors

Inhibitors Comment Organism Structure
aniline
-
 Cereibacter sphaeroides
Ca2+
-
 Cereibacter sphaeroides
hydrazine
-
 Cereibacter sphaeroides
hydroxylamine
-
 Cereibacter sphaeroides
methoxyamine
-
 Cereibacter sphaeroides
methylamine
-
 Cereibacter sphaeroides
Mg2+
-
 Cereibacter sphaeroides
additional information mechanism of inhibition Cereibacter sphaeroides
additional information
-
 Triticum aestivum
NH4+
-
 Cereibacter sphaeroides
opsopyrroledicarboxylic acid 1 mM, 40% inhibition Cereibacter sphaeroides
opsopyrroledicarboxylic acid
-
 Triticum aestivum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 porphobilinogen
-
 Cereibacter sphaeroides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 gel filtration Cereibacter sphaeroides
36000
-
 1 * 36000, SDS-PAGE in the presence of beta-mercaptoethanol Cereibacter sphaeroides
36000
-
 1 * 36000, SDS-PAGE in the presence and in the absence of 2-mercaptoethanol Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
porphobilinogen Bos taurus
-
 ?
-
 ?
porphobilinogen Triticum aestivum
-
 ?
-
 ?
porphobilinogen Cereibacter sphaeroides
-
 ?
-
 ?
porphobilinogen Spinacia oleracea
-
 ?
-
 ?
porphobilinogen Glycine max
-
 ?
-
 ?
porphobilinogen Cereibacter sphaeroides NCIB 8253
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Cereibacter sphaeroides
-
 N.C.I.B. 8253
-
Cereibacter sphaeroides NCIB 8253
-
 N.C.I.B. 8253
-
Glycine max
-
 soya-bean
-
Spinacia oleracea
-
 spinach
-
Triticum aestivum
-
 wheat
-

Purification (Commentary)

Purification (Comment) Organism
-
 Cereibacter sphaeroides

Source Tissue

Source Tissue Comment Organism Textmining
callus
-
 Bos taurus
-
callus
-
 Glycine max
-
germ
-
 Triticum aestivum
-
leaf
-
 Spinacia oleracea
-
liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Cereibacter sphaeroides
0.45
-
-
 Cereibacter sphaeroides

Storage Stability

Storage Stability Organism
-20°C, stable for over 1 year Cereibacter sphaeroides
0°C, stable in solution at pH 7.5 and 9.5 for 3 weeks Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4 porphobilinogen in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 Bos taurus uroporphyrinogen III + 4 NH3
-
 ?
4 porphobilinogen in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 Triticum aestivum uroporphyrinogen III + 4 NH3
-
 ?
4 porphobilinogen in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 Cereibacter sphaeroides uroporphyrinogen III + 4 NH3
-
 ?
4 porphobilinogen in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 Spinacia oleracea uroporphyrinogen III + 4 NH3
-
 ?
4 porphobilinogen in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 Glycine max uroporphyrinogen III + 4 NH3
-
 ?
4 porphobilinogen in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 Cereibacter sphaeroides NCIB 8253 uroporphyrinogen III + 4 NH3
-
 ?
4 porphobilinogen + H2O
-
 Bos taurus hydroxylmethylbilane + 4 NH3
-
 ?
4 porphobilinogen + H2O
-
 Triticum aestivum hydroxylmethylbilane + 4 NH3
-
 ?
4 porphobilinogen + H2O
-
 Spinacia oleracea hydroxylmethylbilane + 4 NH3
-
 ?
4 porphobilinogen + H2O
-
 Glycine max hydroxylmethylbilane + 4 NH3
-
 ?
4 porphobilinogen + H2O
-
 Cereibacter sphaeroides hydroxylmethylbilane + 4 NH3 at pH 8.2 ?
4 porphobilinogen + H2O
-
 Cereibacter sphaeroides hydroxylmethylbilane + 4 NH3 uroporphyrinogen I ?
4 porphobilinogen + H2O
-
 Cereibacter sphaeroides NCIB 8253 hydroxylmethylbilane + 4 NH3 at pH 8.2 ?
4 porphobilinogen + H2O
-
 Cereibacter sphaeroides NCIB 8253 hydroxylmethylbilane + 4 NH3 uroporphyrinogen I ?
porphobilinogen
-
 Bos taurus ?
-
 ?
porphobilinogen
-
 Triticum aestivum ?
-
 ?
porphobilinogen
-
 Cereibacter sphaeroides ?
-
 ?
porphobilinogen
-
 Spinacia oleracea ?
-
 ?
porphobilinogen
-
 Glycine max ?
-
 ?
porphobilinogen
-
 Cereibacter sphaeroides NCIB 8253 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 36000, SDS-PAGE in the presence of beta-mercaptoethanol Cereibacter sphaeroides
monomer 1 * 36000, SDS-PAGE in the presence and in the absence of 2-mercaptoethanol Cereibacter sphaeroides

Synonyms

Synonyms Comment Organism
porphobilinogen deaminase
-
 Cereibacter sphaeroides
uroporphyrinogen I synthetase
-
 Cereibacter sphaeroides
uroporphyrinogen I synthetase
-
 Spinacia oleracea
uroporphyrinogen synthetase
-
 Cereibacter sphaeroides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Cereibacter sphaeroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8 8 0.1-0.4 mM porphobilinogen Cereibacter sphaeroides

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9.2 50% of maximal activity at pH 6.5 and pH 9.2 Cereibacter sphaeroides