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Literature summary for 2.5.1.61 extracted from

  • Jordan, P.M.; Shemin, D.
    Purification and properties of uroporphyrinogen I synthetase from Rhodopseudomonas spheroides (1973), J. Biol. Chem., 248, 1019-1024.
    View publication on PubMed

General Stability

General Stability Organism
enzyme is most stable in 0.3 M LiBr, 0.1 M 2-mercaptoethanol, 30% glycerol, 0.15 M Tris chloride, pH 8.0 to 9.0, or 0.3 M (NH4)2SO4 Cereibacter sphaeroides

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ CaCl2 Cereibacter sphaeroides
Fe2+ 0.1 mM FeSO4 Cereibacter sphaeroides
Fe3+ 0.1 mM FeCl3 Cereibacter sphaeroides
Hg2+ HgCl2, strong Cereibacter sphaeroides
Iodine
-
Cereibacter sphaeroides
Mg2+ MgCl2 Cereibacter sphaeroides
Mn2+ MnCl2 Cereibacter sphaeroides
N-ethylmaleimide
-
Cereibacter sphaeroides
p-chloromercuribenzoate strong Cereibacter sphaeroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.013
-
Hydroxymethylbilane
-
Cereibacter sphaeroides
0.02
-
porphobilinogen 0.1 mM porphobilinogen and 5 mM NaBH4 Cereibacter sphaeroides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35500
-
gel filtration Cereibacter sphaeroides
36000
-
1 * 36000, SDS-PAGE in the presence of beta-mercaptoethanol Cereibacter sphaeroides
39000
-
sucrose density gradient centrifugation Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
porphobilinogen Cereibacter sphaeroides
-
?
-
?

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
ATCC 11167
-

Purification (Commentary)

Purification (Comment) Organism
-
Cereibacter sphaeroides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Cereibacter sphaeroides

Storage Stability

Storage Stability Organism
stored in 0.1 M Tris chloride, pH 8.5, containing 30% glycerol and 0.01 M 2-mercaptoethanol. Under these conditions 50% loss of activity after 3 weeks Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4 porphobilinogen in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 Cereibacter sphaeroides uroporphyrinogen III + 4 NH3
-
?
4 porphobilinogen + H2O
-
Cereibacter sphaeroides hydroxylmethylbilane + 4 NH3
-
?
porphobilinogen
-
Cereibacter sphaeroides ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 36000, SDS-PAGE in the presence of beta-mercaptoethanol Cereibacter sphaeroides

Synonyms

Synonyms Comment Organism
porphobilinogen deaminase
-
Cereibacter sphaeroides
uroporphyrinogen I synthetase
-
Cereibacter sphaeroides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Cereibacter sphaeroides

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
during early stages of purification enzyme may be heated with no loss of activity. Inactivation is quite rapid above Cereibacter sphaeroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
phosphate buffer and Tris buffer Cereibacter sphaeroides

pH Range

pH Minimum pH Maximum Comment Organism
6 8 pH 6.0: enzyme is rapidly and irreversibly inactivated below, pH 8.0: about 50% of maximal activity Cereibacter sphaeroides