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Literature summary for 2.5.1.60 extracted from

  • Wu, Y.; Waldmann, H.; Reents, R.; Ebetino, F.H.; Goody, R.S.; Alexandrov, K.
    A protein fluorescence amplifier: continuous fluorometric assay for Rab geranylgeranyltransferase (2006), Chembiochem, 7, 1859-1861.
    View publication on PubMed
Search for more literature for 2.5.1.60

Inhibitors

Inhibitors Comment Organism Structure
NE10790 a weak bisphosphonate inhibitor Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranylgeranyl diphosphate + Rab protein Rattus norvegicus
-
 S-geranylgeranyl-Rab protein + diphosphate
-
 ?
additional information Rattus norvegicus RabGGTase, which prenylates more than 60 members of the Rab GTPase family, can act on its protein substrates only when they are complexed to an additional factor termed REP, Rab escort protein ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 development of a continuous fluorometric assay using a protein fluorescence amplifier, overview Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranylgeranyl diphosphate + Rab protein
-
 Rattus norvegicus S-geranylgeranyl-Rab protein + diphosphate
-
 ?
geranylgeranyl diphosphate + Rab7 protein the N-terminal Cys207 is preferred to Cys205, activity with Rab7 mutants, overview Rattus norvegicus S-geranylgeranyl-Rab7 protein + diphosphate
-
 ?
additional information RabGGTase, which prenylates more than 60 members of the Rab GTPase family, can act on its protein substrates only when they are complexed to an additional factor termed REP, Rab escort protein Rattus norvegicus ?
-
 ?
[3,7,11-trimethyl-12-(7-nitrobenzo[1,2,5]oxadiazo-4-ylamino)dodeca-2,6,10-trien-1] diphosphate + Rab7 protein i.e. NBD-FPP, a fluorescent analogue of geranylgeranyl diphosphate, which is a lipid donor for RabGGTase Rattus norvegicus ? product is mono-NBD-farnesylated Rab7:REP-1 complex ?

Synonyms

Synonyms Comment Organism
Rab geranylgeranyltransferase
-
 Rattus norvegicus
RabGGTase
-
 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.013
-
 Rab protein pH 7.2, 37°C Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
additional information RabGGTase can act on its protein substrates only when they are complexed to an additional factor termed REP, Rab escort protein Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information inhibition kinetics Rattus norvegicus