Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.60 extracted from

  • Witter, D.J.; Poulter, C.D.
    Yeast geranylgeranyltransferase type-II: steady state kinetic studies of the recombinant enzyme (1996), Biochemistry, 35, 10454-10463.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Nonidet P-40 in assay requirement Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
Zn2+ at concentrations above 0.01 mM Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0011
-
Ypt1p pH 7.3, 30°C Saccharomyces cerevisiae
0.0016
-
geranylgeranyl diphosphate pH 7.3, 30°C Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement, optimal concentration at 8 mM Saccharomyces cerevisiae
Zn2+ requirement, optimal concentration at 0.008 mM, zinc content: 0.7-0.12 mol/mol enzyme Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae JRY1550
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Saccharomyces cerevisiae

Reaction

Reaction Comment Organism Reaction ID
geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate enzyme is unique in the protein prenyltransferase family, consisting protein farnesyltransferase, EC 2.5.1.58, protein geranylgeranyltransferase type I, EC 2.5.1.59 and Rab geranylgeranyltransferase Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0082
-
-
Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranylgeranyl diphosphate + protein-cysteine enzyme attaches geranylgeranyl groups to two C-terminal cysteines in Ras-related GTPases of a single family, the Rab family, Ypt/Sec4 in lower eukaryotes, that terminate in XXCC, XCXC and CCXX motifs, reaction only if Rab is bound to a carrier protein termed Rab escort protein, REP Saccharomyces cerevisiae S-geranylgeranyl-protein + diphosphate
-
?
geranylgeranyl diphosphate + protein-cysteine enzyme attaches geranylgeranyl groups to two C-terminal cysteines in Ras-related GTPases of a single family, the Rab family, Ypt/Sec4 in lower eukaryotes, that terminate in XXCC, XCXC and CCXX motifs, reaction only if Rab is bound to a carrier protein termed Rab escort protein, REP Saccharomyces cerevisiae JRY1550 S-geranylgeranyl-protein + diphosphate
-
?
geranylgeranyl diphosphate + Ypt1p yeast enzyme catalyses the prenylation of Ypt1p in the presence of an escort protein, Msi4p Saccharomyces cerevisiae ?
-
?
geranylgeranyl diphosphate + Ypt1p yeast enzyme catalyses the prenylation of Ypt1p in the presence of an escort protein, Msi4p Saccharomyces cerevisiae JRY1550 ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
Saccharomyces cerevisiae