Application | Comment | Organism |
---|---|---|
drug development | the product S-adenosyl-L-methionine is an effective cure for liver disease, depressive syndromes, and osteoarthritis. A multiple-copy integrative plasmid pYMIKP-SAM2 is introduced into the chromosome of wild-type Saccharomyces cerevisiae strain ZJU001 to construct the recombined strain R1-ZJU001. The recombinant yeast exhibits higher enzymatic activity of methionine adenosyltransferase and improved S-adenosyl-L-methionine biosynthesis. With a three-phase fed-batch strategy in 15-liter bench-top fermentor, 8.81 g/l S-adenosyl-L-methionine is achieved after 52 h cultivation of R1-ZJU001, about 27.1 % increase over its parent strain ZJU001, whereas the S-adenosyl-L-methionine content is also improved from 64.6 mg/g dry cell weight to 91.0 mg/g dry cell weight | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
a multiple-copy integrative plasmid pYMIKP-SAM2 is introduced into the chromosome of wild-type Saccharomyces cerevisiae strain ZJU001 to construct the recombined strain R1-ZJU001 | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + H2O | Saccharomyces cerevisiae | the product S-adenosyl-L-methionine plays important roles in trans-methylation, transsulfuration, and polyamine synthesis in all living cells | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? | |
ATP + L-methionine + H2O | Saccharomyces cerevisiae BY4741 | the product S-adenosyl-L-methionine plays important roles in trans-methylation, transsulfuration, and polyamine synthesis in all living cells | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P19358 | S288c-derivative laboratory strain | - |
Saccharomyces cerevisiae BY4741 | P19358 | S288c-derivative laboratory strain | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + H2O | - |
Saccharomyces cerevisiae | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? | |
ATP + L-methionine + H2O | the product S-adenosyl-L-methionine plays important roles in trans-methylation, transsulfuration, and polyamine synthesis in all living cells | Saccharomyces cerevisiae | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? | |
ATP + L-methionine + H2O | - |
Saccharomyces cerevisiae BY4741 | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? | |
ATP + L-methionine + H2O | the product S-adenosyl-L-methionine plays important roles in trans-methylation, transsulfuration, and polyamine synthesis in all living cells | Saccharomyces cerevisiae BY4741 | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SAM2 | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
metabolism | the product S-adenosyl-L-methionine plays important roles in trans-methylation, transsulfuration, and polyamine synthesis in all living cells | Saccharomyces cerevisiae |