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Literature summary for 2.5.1.6 extracted from

  • Zhao, W.; Shi, F.; Hang, B.; Huang, L.; Cai, J.; Xu, Z.
    The improvement of SAM accumulation by integrating the endogenous methionine adenosyltransferase gene SAM2 in genome of the industrial Saccharomyces cerevisiae strain (2016), Appl. Biochem. Biotechnol., 178, 1263-1272 .
    View publication on PubMed

Application

Application Comment Organism
drug development the product S-adenosyl-L-methionine is an effective cure for liver disease, depressive syndromes, and osteoarthritis. A multiple-copy integrative plasmid pYMIKP-SAM2 is introduced into the chromosome of wild-type Saccharomyces cerevisiae strain ZJU001 to construct the recombined strain R1-ZJU001. The recombinant yeast exhibits higher enzymatic activity of methionine adenosyltransferase and improved S-adenosyl-L-methionine biosynthesis. With a three-phase fed-batch strategy in 15-liter bench-top fermentor, 8.81 g/l S-adenosyl-L-methionine is achieved after 52 h cultivation of R1-ZJU001, about 27.1 % increase over its parent strain ZJU001, whereas the S-adenosyl-L-methionine content is also improved from 64.6 mg/g dry cell weight to 91.0 mg/g dry cell weight Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
a multiple-copy integrative plasmid pYMIKP-SAM2 is introduced into the chromosome of wild-type Saccharomyces cerevisiae strain ZJU001 to construct the recombined strain R1-ZJU001 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-methionine + H2O Saccharomyces cerevisiae the product S-adenosyl-L-methionine plays important roles in trans-methylation, transsulfuration, and polyamine synthesis in all living cells phosphate + diphosphate + S-adenosyl-L-methionine
-
?
ATP + L-methionine + H2O Saccharomyces cerevisiae BY4741 the product S-adenosyl-L-methionine plays important roles in trans-methylation, transsulfuration, and polyamine synthesis in all living cells phosphate + diphosphate + S-adenosyl-L-methionine
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P19358 S288c-derivative laboratory strain
-
Saccharomyces cerevisiae BY4741 P19358 S288c-derivative laboratory strain
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-methionine + H2O
-
Saccharomyces cerevisiae phosphate + diphosphate + S-adenosyl-L-methionine
-
?
ATP + L-methionine + H2O the product S-adenosyl-L-methionine plays important roles in trans-methylation, transsulfuration, and polyamine synthesis in all living cells Saccharomyces cerevisiae phosphate + diphosphate + S-adenosyl-L-methionine
-
?
ATP + L-methionine + H2O
-
Saccharomyces cerevisiae BY4741 phosphate + diphosphate + S-adenosyl-L-methionine
-
?
ATP + L-methionine + H2O the product S-adenosyl-L-methionine plays important roles in trans-methylation, transsulfuration, and polyamine synthesis in all living cells Saccharomyces cerevisiae BY4741 phosphate + diphosphate + S-adenosyl-L-methionine
-
?

Synonyms

Synonyms Comment Organism
SAM2
-
Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Saccharomyces cerevisiae

General Information

General Information Comment Organism
metabolism the product S-adenosyl-L-methionine plays important roles in trans-methylation, transsulfuration, and polyamine synthesis in all living cells Saccharomyces cerevisiae