Protein Variants | Comment | Organism |
---|---|---|
E70S | the mutant enzyme lacks appreciable activity in the presence of non-native methionine surrogates (3.2% S-(-)-methioninol, 240 min versus 25% L-Met, 20 min) | Homo sapiens |
K289F | the mutant enzyme lacks selectivity (33% (3S)-3-amino-5-(methylthio)pentan-2-ol versus 30% L-Met, 240 min) | Homo sapiens |
K289L | 160fold inversion of the enzyme (hMAT2A) selectivity index for a non-native methionine analogue over the native substrate L-methionine. Structure elucidation of K289L reveales the mutant to be folded normally with minor observed repacking within the modified substrate pocket. It is an example of exchanging L-Met terminal carboxylate/amine recognition elements within the hMAT2A active-site to enable non-native bioorthgonal substrate utilization | Homo sapiens |
K289S | mutant displays selectivity toward (3S)-3-amino-5-(methylthio)pentan-2-ol (38%, 240 min) over L-Met (14%, 240 min) | Homo sapiens |
K289T | mutant displays selectivity toward (3S)-3-amino-5-(methylthio)pentan-2-ol (14%, 240 min) over L-Met (2.5%, 240 min) | Homo sapiens |
Q113D | mutant displays selectivity toward (3S)-3-amino-5-(methylthio)pentan-2-ol (39%, 240 min) over L-Met (13%, 240 min) | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.047 | - |
L-methionine | pH 8.0, 37°C, mutant enzyme Q113D | Homo sapiens | |
0.078 | - |
L-methionine | pH 8.0, 37°C, mutant enzyme K289L | Homo sapiens | |
0.14 | - |
L-methionine | pH 8.0, 37°C, wild-type enzyme | Homo sapiens | |
0.39 | - |
S-(-)-methioninol | pH 8.0, 37°C, mutant enzyme Q113D | Homo sapiens | |
1 | - |
S-(-)-methioninol | pH 8.0, 37°C, mutant enzyme K289L | Homo sapiens | |
2.4 | - |
S-(-)-methioninol | pH 8.0, 37°C, wild-type enzyme | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + H2O | Homo sapiens | - |
phosphate + diphosphate + S-adenosyl-L-methionine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P31153 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (3S)-3-amino-5-(methylthio)pentan-2-ol + H2O | - |
Homo sapiens | phosphate + diphosphate + ? | - |
? | |
ATP + L-methionine + H2O | - |
Homo sapiens | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? | |
ATP + S-(-)-methioninol + H2O | - |
Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
methionine adenosyltransferase II-alpha | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
L-methionine | pH 8.0, 37°C, mutant enzyme K289L | Homo sapiens | |
0.02 | - |
L-methionine | pH 8.0, 37°C, mutant enzyme Q113D | Homo sapiens | |
0.055 | - |
S-(-)-methioninol | pH 8.0, 37°C, mutant enzyme Q113D | Homo sapiens | |
0.068 | - |
S-(-)-methioninol | pH 8.0, 37°C, mutant enzyme K289L | Homo sapiens | |
0.183 | - |
S-(-)-methioninol | pH 8.0, 37°C, wild-type enzyme | Homo sapiens | |
0.43 | - |
L-methionine | pH 8.0, 37°C, wild-type enzyme | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.076 | - |
S-(-)-methioninol | pH 8.0, 37°C, wild-type enzyme | Homo sapiens | |
0.141 | - |
S-(-)-methioninol | pH 8.0, 37°C, mutant enzyme Q113D | Homo sapiens | |
0.167 | - |
L-methionine | pH 8.0, 37°C, mutant enzyme K289L | Homo sapiens | |
0.43 | - |
L-methionine | pH 8.0, 37°C, mutant enzyme Q113D | Homo sapiens | |
0.68 | - |
S-(-)-methioninol | pH 8.0, 37°C, mutant enzyme K289L | Homo sapiens | |
3.07 | - |
L-methionine | pH 8.0, 37°C, wild-type enzyme | Homo sapiens |