Literature summary for 2.5.1.6 extracted from

Schlesier, J.; Siegrist, J.; Gerhardt, S.; Erb, A.; Blaesi, S.; Richter, M.; Einsle, O.; Andexer, J.N.
Structural and functional characterisation of the methionine adenosyltransferase from Thermococcus kodakarensis (2013), BMC Struct. Biol., 13, 22.

Search for more literature for 2.5.1.6

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Thermococcus kodakarensis

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 22% (w/v) polyethylene glycol 3350 and 0.2 M sodium citrate	Thermococcus kodakarensis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.31	-	L-methionine	at pH 8.0 and 37°C	Thermococcus kodakarensis
6.54	-	ATP	at pH 8.0 and 37°C	Thermococcus kodakarensis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
44300	-	-	Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + L-methionine + H2O	Thermococcus kodakarensis	-	phosphate + diphosphate + S-adenosyl-L-methionine	-	?

Organism

Organism	UniProt	Comment	Textmining
Thermococcus kodakarensis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
nickel affinity column chromatography	Thermococcus kodakarensis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-methionine + H2O	-	Thermococcus kodakarensis	phosphate + diphosphate + S-adenosyl-L-methionine	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 40000-55000, SDS-PAGE	Thermococcus kodakarensis
homodimer	2 * 44300, calculated from amino acid sequence	Thermococcus kodakarensis

Synonyms

Synonyms	Comment	Organism
MAT	-	Thermococcus kodakarensis
methionine adenosyltransferase	-	Thermococcus kodakarensis
MetK	-	Thermococcus kodakarensis
S-adenosylmethionine synthase	-	Thermococcus kodakarensis

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Release 2023.1 (January 2023)