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Literature summary for 2.5.1.6 extracted from

  • Shafqat, N.; Muniz, J.R.; Pilka, E.S.; Papagrigoriou, E.; von Delft, F.; Oppermann, U.; Yue, W.W.
    Insight into S-adenosylmethionine biosynthesis from the crystal structures of the human methionine adenosyltransferase catalytic and regulatory subunits (2013), Biochem. J., 452, 27-36.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method Homo sapiens
sitting drop vapor diffusion method, using Homo sapiens

Protein Variants

Protein Variants Comment Organism
A55D the mutation reduces the enzyme activity by more than 50% Homo sapiens
D258G the mutation reduces the enzyme activity by more than 50% Homo sapiens
I322M the mutation reduces the enzyme activity by more than 50% Homo sapiens
R264H the mutation almost completely abolishes enzyme activity Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-methionine + H2O Homo sapiens
-
 phosphate + diphosphate + S-adenosyl-L-methionine
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P31153 isoform MAT2A
-
Homo sapiens Q00266 isoform MAT1A
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, HiTrap HP-Q column chromatography, and Superdex 200 gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver isoform MAT1A Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-methionine + H2O
-
 Homo sapiens phosphate + diphosphate + S-adenosyl-L-methionine
-
 ?

Subunits

Subunits Comment Organism
homodimer x-ray crystallography Homo sapiens

Synonyms

Synonyms Comment Organism
MAT1A isoform Homo sapiens
MAT2A isoform Homo sapiens
methionine adenosyltransferase
-
 Homo sapiens
S-adenosylmethionine synthetase
-
 Homo sapiens