Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Methanocaldococcus jannaschii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.14 | - |
L-methionine | pH 8.0, 58°C | Methanocaldococcus jannaschii | |
0.25 | - |
ATP | pH 8.0, 58°C | Methanocaldococcus jannaschii | |
1.4 | - |
ITP | pH 8.0, 58°C | Methanocaldococcus jannaschii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | half-maximal activation at 25 mM KCl | Methanocaldococcus jannaschii | |
Mg2+ | no activity is detectable in the absence of Mg2+, and maximal activity is observed at 10 mM MgCl2 when ATP is present at 5 mM, indicating that MgATP is probably the true substrate | Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + H2O | Methanocaldococcus jannaschii | biosynthesis of S-adenosyl-L-methionine | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? | |
ATP + L-methionine + H2O | Methanocaldococcus jannaschii DSM 2661 | biosynthesis of S-adenosyl-L-methionine | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | Q58605 | - |
- |
Methanocaldococcus jannaschii DSM 2661 | Q58605 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + H2O | biosynthesis of S-adenosyl-L-methionine | Methanocaldococcus jannaschii | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? | |
ATP + L-methionine + H2O | two-step reaction in which reaction of ATP and methionine initially yields the S-adenosyl-L-methionine and tripolyphosphate. The tripolyphosphate remains enzyme-bound and is cleaved to diphosphate and phosphate before product release | Methanocaldococcus jannaschii | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? | |
ATP + L-methionine + H2O | biosynthesis of S-adenosyl-L-methionine | Methanocaldococcus jannaschii DSM 2661 | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? | |
ATP + L-methionine + H2O | two-step reaction in which reaction of ATP and methionine initially yields the S-adenosyl-L-methionine and tripolyphosphate. The tripolyphosphate remains enzyme-bound and is cleaved to diphosphate and phosphate before product release | Methanocaldococcus jannaschii DSM 2661 | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? | |
ITP + L-methionine + H2O | - |
Methanocaldococcus jannaschii | ? | - |
? | |
ITP + L-methionine + H2O | - |
Methanocaldococcus jannaschii DSM 2661 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MAT | - |
Methanocaldococcus jannaschii |
MJ1208 | locus name | Methanocaldococcus jannaschii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
58 | - |
assay at | Methanocaldococcus jannaschii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Methanocaldococcus jannaschii |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme is involved in biosynthesis of S-adenosyl-L-methionine | Methanocaldococcus jannaschii |