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Literature summary for 2.5.1.6 extracted from

  • Kamarthapu, V.; Rao, K.V.; Srinivas, P.N.; Reddy, G.B.; Reddy, V.D.
    Structural and kinetic properties of Bacillus subtilis S-adenosylmethionine synthetase expressed in Escherichia coli (2008), Biochim. Biophys. Acta, 1784, 1949-1958.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.26
-
L-methionine in 100 mM Tris-Cl pH 8.0, 20 mM MgCl2, at 37°C Bacillus subtilis
0.92
-
ATP in 100 mM Tris-Cl pH 8.0, 20 mM MgCl2, at 37°C Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 0.02 mM stimulates activity Bacillus subtilis
Co2+ 0.02 mM stimulates activity Bacillus subtilis
K+ 0.2 mM stimulates activity Bacillus subtilis
Mg2+ required for activity Bacillus subtilis
Ni2+ 0.02 mM stimulates activity Bacillus subtilis
Zn2+ 0.02 mM stimulates activity Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
4 * 45000, dimer of dimer, gel filtration Bacillus subtilis
180000
-
gel filtration Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis P54419
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-methionine + H2O
-
Bacillus subtilis phosphate + diphosphate + S-adenosyl-L-methionine
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 45000, dimer of dimer, gel filtration Bacillus subtilis

Synonyms

Synonyms Comment Organism
S-adenosylmethionine synthetase
-
Bacillus subtilis
SAM synthetase
-
Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Bacillus subtilis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 55
-
Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Bacillus subtilis

pH Range

pH Minimum pH Maximum Comment Organism
6 10
-
Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
ATP
-
Bacillus subtilis