Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) cells | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.26 | - |
L-methionine | in 100 mM Tris-Cl pH 8.0, 20 mM MgCl2, at 37°C | Bacillus subtilis | |
0.92 | - |
ATP | in 100 mM Tris-Cl pH 8.0, 20 mM MgCl2, at 37°C | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 0.02 mM stimulates activity | Bacillus subtilis | |
Co2+ | 0.02 mM stimulates activity | Bacillus subtilis | |
K+ | 0.2 mM stimulates activity | Bacillus subtilis | |
Mg2+ | required for activity | Bacillus subtilis | |
Ni2+ | 0.02 mM stimulates activity | Bacillus subtilis | |
Zn2+ | 0.02 mM stimulates activity | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
4 * 45000, dimer of dimer, gel filtration | Bacillus subtilis |
180000 | - |
gel filtration | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P54419 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + H2O | - |
Bacillus subtilis | phosphate + diphosphate + S-adenosyl-L-methionine | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 45000, dimer of dimer, gel filtration | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
S-adenosylmethionine synthetase | - |
Bacillus subtilis |
SAM synthetase | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Bacillus subtilis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 55 | - |
Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | - |
Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Bacillus subtilis |