Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.6 extracted from

  • Perez-Pertejo, Y.; Reguera, R.M.; Garcia-Estrada, C.; Balana-Fouce, R.; Ordonez, D.
    Mutational analysis of methionine adenosyltransferase from Leishmania donovani (2004), Eur. J. Biochem., 271, 2791-2798.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
D121N no basal enzymic activity, little activity in presence of S-adenosyl methionine Leishmania donovani
D166N reduced enzymic activity, little activation by S-adenosyl methionine Leishmania donovani
D19N no basal enzymic activity, little activity in presence of S-adenosyl methionine Leishmania donovani
D249N reduced enzymic activity, little activation by S-adenosyl methionine Leishmania donovani
D277N no enzymic activity Leishmania donovani
D282N reduced enzymic activity, little activation by S-adenosyl methionine Leishmania donovani
F241A no enzymic activity, but hydrolysis of triphosphate Leishmania donovani
H17A no basal enzymic activity, little activity in presence of S-adenosyl methionine Leishmania donovani
H17N no basal enzymic activity, little activity in presence of S-adenosyl methionine Leishmania donovani
K168A no enzymic activity Leishmania donovani
K256A no activation by S-adenosyl methionine Leishmania donovani
K276A no enzymic activity Leishmania donovani
K280A enhanced enzymic activity, little activation by S-adenosyl methionine Leishmania donovani
R255L no enzymic activity Leishmania donovani

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
L-methionine mutant D249N, pH 8.0 Leishmania donovani
0.036
-
L-methionine mutant D166N, pH 8.0 Leishmania donovani
0.038
-
L-methionine mutant D19N, presence of 0.05 mM S-adenosyl methionine, pH 8.0 Leishmania donovani
0.04
-
L-methionine wild-type, pH 8.0 Leishmania donovani
0.041
-
L-methionine mutant K280A, pH 8.0 Leishmania donovani
0.045
-
L-methionine mutant D121N, presence of 0.05 mM S-adenosyl methionine, pH 8.0 Leishmania donovani
0.05
-
L-methionine mutant D249N, presence of 0.05 mM S-adenosyl methionine, pH 8.0 Leishmania donovani
0.074
-
L-methionine mutant K280A, presence of 0.05 mM S-adenosyl methionine, pH 8.0 Leishmania donovani
0.137
-
L-methionine mutant D282N, presence of 0.05 mM S-adenosyl methionine, pH 8.0 Leishmania donovani
0.137
-
L-methionine mutant H17N, presence of 0.05 mM S-adenosyl methionine, pH 8.0 Leishmania donovani
0.141
-
L-methionine mutant D282N, pH 8.0 Leishmania donovani
0.147
-
L-methionine mutant D166N, presence of 0.05 mM S-adenosyl methionine, pH 8.0 Leishmania donovani
0.17
-
L-methionine mutant K256A, pH 8.0 Leishmania donovani
0.17
-
L-methionine mutant K256A, presence of 0.05 mM S-adenosyl methionine, pH 8.0 Leishmania donovani
0.176
-
L-methionine mutant H17A, presence of 0.05 mM S-adenosyl methionine, pH 8.0 Leishmania donovani
0.287
-
L-methionine wild-type, presence of 0.05 mM S-adenosyl methionine, pH 8.0 Leishmania donovani

Organism

Organism UniProt Comment Textmining
Leishmania donovani Q9U6Q8
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
66
-
mutant D121N, presence of 0.05 mM S-adenosyl methionine, pH 8.0 Leishmania donovani
108
-
mutant D166N, pH 8.0 Leishmania donovani
108
-
mutant D19N, presence of 0.05 mM S-adenosyl methionine, pH 8.0 Leishmania donovani
108
-
mutant D282N, pH 8.0 Leishmania donovani
138
-
mutant D249N, pH 8.0 Leishmania donovani
210
-
mutant H17A, presence of 0.05 mM S-adenosyl methionine, pH 8.0 Leishmania donovani
222
-
wild-type, pH 8.0 Leishmania donovani
240
-
mutant D282N, presence of 0.05 mM S-adenosyl methionine, pH 8.0 Leishmania donovani
252
-
mutant K256A, pH 8.0 Leishmania donovani
252
-
mutant K256A, presence of 0.05 mM S-adenosyl methionine, pH 8.0 Leishmania donovani
318
-
mutant K280A, pH 8.0 Leishmania donovani
330
-
mutant D249N, presence of 0.05 mM S-adenosyl methionine, pH 8.0 Leishmania donovani
720
-
mutant K280A, presence of 0.05 mM S-adenosyl methionine, pH 8.0 Leishmania donovani
792
-
mutant D166N, presence of 0.05 mM S-adenosyl methionine, pH 8.0 Leishmania donovani
960
-
mutant H17N, presence of 0.05 mM S-adenosyl methionine, pH 8.0 Leishmania donovani
3000
-
wild-type, presence of 0.05 mM S-adenosyl methionine, pH 8.0 Leishmania donovani

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-methionine + H2O
-
Leishmania donovani phosphate + diphosphate + S-adenosyl-L-methionine
-
?