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Literature summary for 2.5.1.6 extracted from

  • Lu, Z.J.; Markham, G.D.
    Enzymatic properties of S-adenosylmethionine synthetase from the archaeon Methanococcus jannaschii (2002), J. Biol. Chem., 277, 16624-16631.
    View publication on PubMed
Search for more literature for 2.5.1.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)codon plus/pMJ1208-strain with a decahistidine tag on the N-terminus Methanocaldococcus jannaschii

Inhibitors

Inhibitors Comment Organism Structure
GTP competitive with respect to ATP and noncompetitive with L-methionine Methanocaldococcus jannaschii
L-ethionine competitive with respect to methionine for S-adenosylmethionine formation and noncompetitive with respect to ATP Methanocaldococcus jannaschii
additional information no inhibition with cycloleucine, L-homocysteine, L-norleucine, L-cis-2-amino-4-methoxy-3-butenoic acid, S-adenosylhomocysteine, 5'-methylthioadenosine, sinefungin Methanocaldococcus jannaschii
phosphate competitive toward both ATP and methionine Methanocaldococcus jannaschii
S-adenosylmethionine noncompetitive inhibitor with respect to ATP and methionine Methanocaldococcus jannaschii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
 ATP saturated with KCl Methanocaldococcus jannaschii
0.24
-
 L-methionine
-
 Methanocaldococcus jannaschii
0.26
-
 ATP
-
 Methanocaldococcus jannaschii
0.3
-
 L-methionine saturated with KCl Methanocaldococcus jannaschii
0.4
-
 CTP
-
 Methanocaldococcus jannaschii
0.62
-
 GTP
-
 Methanocaldococcus jannaschii
0.74
-
 L-ethionine
-
 Methanocaldococcus jannaschii
1.1
-
 L-methionine
-
 Methanocaldococcus jannaschii
1.4
-
 ATP
-
 Methanocaldococcus jannaschii
2.2
-
 UTP
-
 Methanocaldococcus jannaschii
2.6
-
 L-methionine methyl esther
-
 Methanocaldococcus jannaschii
3.5
-
 D-methionine
-
 Methanocaldococcus jannaschii

Metals/Ions

Metals/Ions Comment Organism Structure
K+ enhances Kcat and decrees Km values for both substrates Methanocaldococcus jannaschii
Mg2+ absolute requirement Methanocaldococcus jannaschii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 2 * 45000 Methanocaldococcus jannaschii
86000
-
 recombinant enzyme, gel filtration Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-methionine + H2O Methanocaldococcus jannaschii
-
 S-adenosyl-L-methionine + phosphate + diphosphate
-
 ir

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii
-
 hypertermophilic archaeon
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme purified by a method that includes Ni2+-His binding resin, HiLoad Q-Sepharose and Sephadex-200 chromatography Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-deoxy-ATP + L-methionine + H2O
-
 Methanocaldococcus jannaschii ?
-
 ir
3'-deoxy-ATP + L-methionine + H2O
-
 Methanocaldococcus jannaschii ?
-
 ir
ATP + D-methionine + H2O
-
 Methanocaldococcus jannaschii S-adenosyl-D-methionine + phosphate + diphosphate
-
 ?
ATP + L-ethionine + H2O
-
 Methanocaldococcus jannaschii S-adenosyl-L-ethionine + phosphate + diphosphate
-
 ?
ATP + L-methionine + H2O
-
 Methanocaldococcus jannaschii S-adenosyl-L-methionine + phosphate + diphosphate
-
 ir
ATP + L-methionine methyl ester + H2O
-
 Methanocaldococcus jannaschii S-adenosyl-L-methionine methyl ester + phosphate + diphosphate
-
 ?
CTP + L-methionine + H2O
-
 Methanocaldococcus jannaschii S-cytosyl-L-methionine + phosphate + diphosphate
-
 ?
GTP + L-methionine + H2O
-
 Methanocaldococcus jannaschii S-guanosyl-L-methionine + phosphate + diphosphate
-
 ?
additional information no activity with methional, methioninol, 3-methylthiopropylamine Methanocaldococcus jannaschii ?
-
 ?
UTP + L-methionine + H2O
-
 Methanocaldococcus jannaschii S-urasyl-L-methionine + phosphate + diphosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 45000 Methanocaldococcus jannaschii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 optimal growth temperature for Methanococcus jannaschii: 87°C Methanocaldococcus jannaschii