Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)codon plus/pMJ1208-strain with a decahistidine tag on the N-terminus | Methanocaldococcus jannaschii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GTP | competitive with respect to ATP and noncompetitive with L-methionine | Methanocaldococcus jannaschii | |
L-ethionine | competitive with respect to methionine for S-adenosylmethionine formation and noncompetitive with respect to ATP | Methanocaldococcus jannaschii | |
additional information | no inhibition with cycloleucine, L-homocysteine, L-norleucine, L-cis-2-amino-4-methoxy-3-butenoic acid, S-adenosylhomocysteine, 5'-methylthioadenosine, sinefungin | Methanocaldococcus jannaschii | |
phosphate | competitive toward both ATP and methionine | Methanocaldococcus jannaschii | |
S-adenosylmethionine | noncompetitive inhibitor with respect to ATP and methionine | Methanocaldococcus jannaschii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
ATP | saturated with KCl | Methanocaldococcus jannaschii | |
0.24 | - |
L-methionine | - |
Methanocaldococcus jannaschii | |
0.26 | - |
ATP | - |
Methanocaldococcus jannaschii | |
0.3 | - |
L-methionine | saturated with KCl | Methanocaldococcus jannaschii | |
0.4 | - |
CTP | - |
Methanocaldococcus jannaschii | |
0.62 | - |
GTP | - |
Methanocaldococcus jannaschii | |
0.74 | - |
L-ethionine | - |
Methanocaldococcus jannaschii | |
1.1 | - |
L-methionine | - |
Methanocaldococcus jannaschii | |
1.4 | - |
ATP | - |
Methanocaldococcus jannaschii | |
2.2 | - |
UTP | - |
Methanocaldococcus jannaschii | |
2.6 | - |
L-methionine methyl esther | - |
Methanocaldococcus jannaschii | |
3.5 | - |
D-methionine | - |
Methanocaldococcus jannaschii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | enhances Kcat and decrees Km values for both substrates | Methanocaldococcus jannaschii | |
Mg2+ | absolute requirement | Methanocaldococcus jannaschii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
2 * 45000 | Methanocaldococcus jannaschii |
86000 | - |
recombinant enzyme, gel filtration | Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + H2O | Methanocaldococcus jannaschii | - |
S-adenosyl-L-methionine + phosphate + diphosphate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | - |
hypertermophilic archaeon | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme purified by a method that includes Ni2+-His binding resin, HiLoad Q-Sepharose and Sephadex-200 chromatography | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-deoxy-ATP + L-methionine + H2O | - |
Methanocaldococcus jannaschii | ? | - |
ir | |
3'-deoxy-ATP + L-methionine + H2O | - |
Methanocaldococcus jannaschii | ? | - |
ir | |
ATP + D-methionine + H2O | - |
Methanocaldococcus jannaschii | S-adenosyl-D-methionine + phosphate + diphosphate | - |
? | |
ATP + L-ethionine + H2O | - |
Methanocaldococcus jannaschii | S-adenosyl-L-ethionine + phosphate + diphosphate | - |
? | |
ATP + L-methionine + H2O | - |
Methanocaldococcus jannaschii | S-adenosyl-L-methionine + phosphate + diphosphate | - |
ir | |
ATP + L-methionine methyl ester + H2O | - |
Methanocaldococcus jannaschii | S-adenosyl-L-methionine methyl ester + phosphate + diphosphate | - |
? | |
CTP + L-methionine + H2O | - |
Methanocaldococcus jannaschii | S-cytosyl-L-methionine + phosphate + diphosphate | - |
? | |
GTP + L-methionine + H2O | - |
Methanocaldococcus jannaschii | S-guanosyl-L-methionine + phosphate + diphosphate | - |
? | |
additional information | no activity with methional, methioninol, 3-methylthiopropylamine | Methanocaldococcus jannaschii | ? | - |
? | |
UTP + L-methionine + H2O | - |
Methanocaldococcus jannaschii | S-urasyl-L-methionine + phosphate + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 45000 | Methanocaldococcus jannaschii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
optimal growth temperature for Methanococcus jannaschii: 87°C | Methanocaldococcus jannaschii |