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Literature summary for 2.5.1.58 extracted from

  • Pais, J.E.; Bowers, K.E.; Stoddard, A.K.; Fierke, C.A.
    A continuous fluorescent assay for protein prenyltransferases measuring diphosphate release (2005), Anal. Biochem., 345, 302-311.
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
continuous fluorescent assay for protein prenyltransferases measuring diphosphate release Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
farnesyl diphosphate + GCVLS
-
Escherichia coli Gly-S-farnesyl-Cys-Val-Leu-Ser + diphosphate
-
?
farnesyl diphosphate + Thr-Lys-Cys-Val-Ile-Leu
-
Escherichia coli Thr-Lys-S-farnesyl-Cys-Val-Ile-Leu + diphosphate
-
?
farnesyl diphosphate + Thr-Lys-Cys-Val-Ile-Met
-
Escherichia coli Thr-Lys-S-farnesyl-Cys-Val-Ile-Met + diphosphate
-
?
farnesyl diphosphate + Thr-Lys-Cys-Val-Ile-Phe
-
Escherichia coli Thr-Lys-S-farnesyl-Cys-Val-Ile-Phe + diphosphate
-
?
farnesyl diphosphate + Thr-Lys-Cys-Val-Ile-Ser
-
Escherichia coli Thr-Lys-S-farnesyl-Cys-Val-Ile-Ser + diphosphate
-
?
farnesyl diphosphate + Thr-Lys-Cys-Val-Leu-Ala
-
Escherichia coli Thr-Lys-S-farnesyl-Cys-Val-Leu-Ala + diphosphate
-
?
farnesyl diphosphate + Thr-Lys-Val-Ile-Phe
-
Escherichia coli ?
-
?

Synonyms

Synonyms Comment Organism
FTase
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0025
-
Thr-Lys-Val-Ile-Phe 25°C, pH 7.8 Escherichia coli
0.08
-
Thr-Lys-Cys-Val-Leu-Ala 25°C, pH 7.8 Escherichia coli
0.1
-
Thr-Lys-Cys-Val-Ile-Met 25°C, pH 7.8 Escherichia coli