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Literature summary for 2.5.1.58 extracted from

  • Schmitt, D.; Callan, K.; Gruissem.W.
    Molecular and biochemical characterization of tomato farnesyl-protein transferase (1996), Plant Physiol., 112, 767-777.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Solanum lycopersicum

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
Solanum lycopersicum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Solanum lycopersicum
Zn2+ required Solanum lycopersicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
gel filtration Solanum lycopersicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
farnesyl diphosphate + protein-cysteine Solanum lycopersicum a possible role for the enzyme during fruit development S-farnesyl protein + diphosphate
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
additional information EDTA-treatment decreases activity Solanum lycopersicum

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum
-
4-week-old hydroponically grown tomato plants, VFNT cherry
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Solanum lycopersicum

Source Tissue

Source Tissue Comment Organism Textmining
fruit decreased activity approximately 10fold between young and mature green fruits, increased activity during fruit development in red ripe fruit Solanum lycopersicum
-
leaf low activity Solanum lycopersicum
-
additional information no significant difference in enzyme activity between dark-grown and light-grown seedlings Solanum lycopersicum
-
root
-
Solanum lycopersicum
-
stem high activity in tissues from young plants Solanum lycopersicum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.08
-
-
Solanum lycopersicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
farnesyl diphosphate + protein-cysteine the specific CAQQ motif of the ANJ1 protein has a low affinity for enzyme in vitro, enzyme specifically farnesylated the RasB-CVIM peptide substrate Solanum lycopersicum diphosphate + S-farnesyl protein
-
?
farnesyl diphosphate + protein-cysteine a possible role for the enzyme during fruit development Solanum lycopersicum S-farnesyl protein + diphosphate
-
?
farnesyl diphosphate + Ras-Cys-Val-Ile-Met best substrate Solanum lycopersicum diphosphate + Ras-S-farnesyl-Cys-Val-Ile-Met
-
?

Subunits

Subunits Comment Organism
heterodimer
-
Solanum lycopersicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Solanum lycopersicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
Solanum lycopersicum