Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Solanum lycopersicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Solanum lycopersicum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Solanum lycopersicum | |
Zn2+ | required | Solanum lycopersicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
gel filtration | Solanum lycopersicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
farnesyl diphosphate + protein-cysteine | Solanum lycopersicum | a possible role for the enzyme during fruit development | S-farnesyl protein + diphosphate | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
additional information | EDTA-treatment decreases activity | Solanum lycopersicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | - |
4-week-old hydroponically grown tomato plants, VFNT cherry | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Solanum lycopersicum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | decreased activity approximately 10fold between young and mature green fruits, increased activity during fruit development in red ripe fruit | Solanum lycopersicum | - |
leaf | low activity | Solanum lycopersicum | - |
additional information | no significant difference in enzyme activity between dark-grown and light-grown seedlings | Solanum lycopersicum | - |
root | - |
Solanum lycopersicum | - |
stem | high activity in tissues from young plants | Solanum lycopersicum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.08 | - |
- |
Solanum lycopersicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
farnesyl diphosphate + protein-cysteine | the specific CAQQ motif of the ANJ1 protein has a low affinity for enzyme in vitro, enzyme specifically farnesylated the RasB-CVIM peptide substrate | Solanum lycopersicum | diphosphate + S-farnesyl protein | - |
? | |
farnesyl diphosphate + protein-cysteine | a possible role for the enzyme during fruit development | Solanum lycopersicum | S-farnesyl protein + diphosphate | - |
? | |
farnesyl diphosphate + Ras-Cys-Val-Ile-Met | best substrate | Solanum lycopersicum | diphosphate + Ras-S-farnesyl-Cys-Val-Ile-Met | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | - |
Solanum lycopersicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Solanum lycopersicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | - |
Solanum lycopersicum |