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Literature summary for 2.5.1.58 extracted from

  • Ryu, K.Y.; Baik, Y.J.; Yang, C.H.
    Purification and characterization of farnesyl protein transferase from bovine testis (1995), J. Biochem. Mol. Biol., 28, 197-203.
No PubMed abstract available
Search for more literature for 2.5.1.58

Activating Compound

Activating Compound Comment Organism Structure
additional information n-octyl beta-D-glycopyranoside stimulates enzyme activity even at concentrations of 0.5% Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Bos taurus
Lys-Lys-Cys-Val-Ile-Met 50% inhibition at 0.0025 mM Bos taurus
sodium deoxycholate
-
 Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Bos taurus
Zn2+ required Bos taurus
Zn2+ zinc metalloenzyme Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 gel filtration Bos taurus

Organic Solvent Stability

Organic Solvent Comment Organism
additional information n-octyl beta-D-glycopyranoside stimulates even at concentrations of 0.5% Bos taurus
additional information EDTA-treatment decreases activity Bos taurus
Triton X-100 and Zwittergent 3-14, no effect on the activity of the enzyme Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
testis
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0207
-
-
 Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
farnesyl diphosphate + protein-cysteine posttranslational lipid modification in which a 15-carbon farnesyl isoprenoid is linked via a thioether bond to specific cysteine residues of proteins, the reactive cysteine is located in the C-terminal Ca1a2X motif in which C is the modified cysteine, a1 and a2 are often an aliphatic residue, and X is Ser, Met, Ala or Gln Bos taurus diphosphate + S-farnesyl protein
-
 ?
farnesyl diphosphate + protein-cysteine substrate: Ras-CVIM and GST-PEP Bos taurus diphosphate + S-farnesyl protein
-
 ?
farnesyl diphosphate + Ras-Cys-Val-Ile-Met
-
 Bos taurus diphosphate + Ras-S-farnesyl-Cys-Val-Ile-Met
-
 ?

Subunits

Subunits Comment Organism
heterodimer
-
 Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
 Bos taurus