Literature summary for 2.5.1.56 extracted from

Ishikawa, M.; Koizumi, S.
Microbial production of N-acetylneuraminic acid by genetically engineered Escherichia coli (2010), Carbohydr. Res., 345, 2605-2609.

Search for more literature for 2.5.1.56

Application

Application	Comment	Organism
synthesis	construction of a recombinant Escherichia coli simultaneously overexpressing GlcNAc 2-epimerase and N-acetylneuraminic acid synthase. The recombinant strain produces 25 g/l N-acetylneuraminic acid in 22 h without the addition of Corynebacterium ammoniagenes cells. For manufacturing on an industrial scale, it is preferable to use unconcentrated culture broth as the source of enzymes. The N-acetylneuraminic acid aldolase gene of the host strain is disrupted yielding a strain which cannot degrade N-acetylneuraminic acid. After a 22 h reaction with 120 g/l N-acetylglucosamine in a 5 l jar fermenter, the culture broth of contains 53 g/l N-acetylneuraminic acid	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Synonyms

Synonyms	Comment	Organism
NeuB	-	Escherichia coli

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Release 2023.1 (January 2023)