Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | divalent metal required, optimal activity with Mn2+ or Mg2+ | Escherichia coli | |
Mn2+ | divalent metal required | Escherichia coli | |
Mn2+ | optimal activity with Mn2+ or Mg2+ | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-D-mannosamine + phosphoenolpyruvate + H2O | Escherichia coli | the enzyme is necessary for the synthesis of N-acetylneuraminate which is required for synthesis of the capsular polysaccharide alpha(2-8)poly-N-acetylneuraminic acid. This polysaccharide is an essential virulence factor of the neuropathogenic bacteria | ? | - |
? | |
N-acetyl-D-mannosamine + phosphoenolpyruvate + H2O | Escherichia coli K1 | the enzyme is necessary for the synthesis of N-acetylneuraminate which is required for synthesis of the capsular polysaccharide alpha(2-8)poly-N-acetylneuraminic acid. This polysaccharide is an essential virulence factor of the neuropathogenic bacteria | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli K1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000416 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-D-mannosamine + phosphoenolpyruvate + H2O | specific for | Escherichia coli | N-acetylneuraminate + phosphate | - |
? | |
N-acetyl-D-mannosamine + phosphoenolpyruvate + H2O | specific for | Escherichia coli K1 | N-acetylneuraminate + phosphate | - |
? | |
N-acetyl-D-mannosamine + phosphoenolpyruvate + H2O | the enzyme is necessary for the synthesis of N-acetylneuraminate which is required for synthesis of the capsular polysaccharide alpha(2-8)poly-N-acetylneuraminic acid. This polysaccharide is an essential virulence factor of the neuropathogenic bacteria | Escherichia coli | ? | - |
? | |
N-acetyl-D-mannosamine + phosphoenolpyruvate + H2O | the enzyme is necessary for the synthesis of N-acetylneuraminate which is required for synthesis of the capsular polysaccharide alpha(2-8)poly-N-acetylneuraminic acid. This polysaccharide is an essential virulence factor of the neuropathogenic bacteria | Escherichia coli K1 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Escherichia coli |