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Literature summary for 2.5.1.55 extracted from

  • Duewel, H.S.; Sheflyan, G.Y.; Woodard, R.W.
    Functional and biochemical characterization of a recombinant 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase from the hyperthermophilic bacterium Aquifex aeolicus (1999), Biochem. Biophys. Res. Commun., 263, 346-351.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Aquifex aeolicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
D-arabinose 5-phosphate pH 7.5, 60°C Aquifex aeolicus
0.015
-
D-arabinose 5-phosphate pH 7.5, 70°C Aquifex aeolicus
0.027
-
D-arabinose 5-phosphate pH 7.5, 80°C Aquifex aeolicus
0.028
-
phosphoenolpyruvate pH 7.5, at 90°C Aquifex aeolicus
0.038
-
phosphoenolpyruvate pH 7.5, at 80°C Aquifex aeolicus
0.043
-
phosphoenolpyruvate pH 7.5, at 60°C or 70°C Aquifex aeolicus
0.074
-
D-arabinose 5-phosphate pH 7.5, 90°C Aquifex aeolicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29736
-
3 * 29736, electrospray ionization mass spectrometry Aquifex aeolicus
97600
-
gel filtration Aquifex aeolicus

Organism

Organism UniProt Comment Textmining
Aquifex aeolicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Aquifex aeolicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.38
-
-
Aquifex aeolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + D-arabinose 5-phosphate
-
Aquifex aeolicus 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate
-
?

Subunits

Subunits Comment Organism
? 3 * 29736, electrospray ionization mass spectrometry Aquifex aeolicus
trimer
-
Aquifex aeolicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
-
Aquifex aeolicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
half-life: 30.3 h Aquifex aeolicus
80
-
half-life: 8.1 h Aquifex aeolicus
90
-
half-life: 1.5 h Aquifex aeolicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.38
-
phosphoenolpyruvate pH 7.5, 60°C Aquifex aeolicus
0.38
-
D-arabinose 5-phosphate pH 7.5, 60°C Aquifex aeolicus
0.87
-
phosphoenolpyruvate pH 7.5, 70°C Aquifex aeolicus
0.87
-
D-arabinose 5-phosphate pH 7.5, 70°C Aquifex aeolicus
1.47
-
phosphoenolpyruvate pH 7.5, 80°C Aquifex aeolicus
1.47
-
D-arabinose 5-phosphate pH 7.5, 80°C Aquifex aeolicus
2
-
phosphoenolpyruvate pH 7.5, 90°C Aquifex aeolicus
2
-
D-arabinose 5-phosphate pH 7.5, 90°C Aquifex aeolicus