Literature summary for 2.5.1.55 extracted from

Krosky, D.J.; Alm, R.; Berg, M.; Carmel, G.; Tummino, P.J.; Xu, B.; Yang, W.
Helicobacter pylori 3-deoxy-D-manno-octulosonate-8-phosphate (KDO-8-P) synthase is a zinc-metalloenzyme (2002), Biochim. Biophys. Acta, 1594, 297-306.

Search for more literature for 2.5.1.55

Activating Compound

Activating Compound	Comment	Organism	Structure
EDTA	1 mM, 40% stimulation	Escherichia coli

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Helicobacter pylori

Inhibitors

Inhibitors	Comment	Organism	Structure
1,10-phenanthroline	-	Escherichia coli
1,10-phenanthroline	IC50: 0.0293 mM	Helicobacter pylori
2,6-pyridine dicarboxylic acid	IC50: 0.0422	Helicobacter pylori
Cu2+	-	Escherichia coli
Cu2+	activity is enhanced below 0.001 mM, inhibitory at higher concentrations	Helicobacter pylori
EDTA	-	Escherichia coli
EDTA	IC50: 0.212 mM	Helicobacter pylori
Zn2+	-	Escherichia coli
Zn2+	mild inhibitor at higher concentrations	Helicobacter pylori

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00443	-	phosphoenolpyruvate	pH 6.5, 25°C	Escherichia coli
0.00593	-	D-arabinose 5-phosphate	pH 6.5, 25°C, Cd2+-enzyme	Helicobacter pylori
0.00648	-	phosphoenolpyruvate	pH 6.5, 25°C, Cd2+-enzyme	Helicobacter pylori
0.0075	-	phosphoenolpyruvate	pH 6.5, 25°C, Zn2+-enzyme	Helicobacter pylori
0.00773	-	D-arabinose 5-phosphate	pH 6.5, 25°C	Escherichia coli
0.0385	-	D-arabinose 5-phosphate	pH 6.5, 25°C, Zn2+-enzyme	Helicobacter pylori

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cd2+	the Zn2+ in the enzyme can be quantitatively replaced by Cd2+ which increases the observed turnover number and decreases the apparent Km-value for D-arabinose-5-phosphate by 6.5fold	Helicobacter pylori
Co2+	stimulates	Helicobacter pylori
Cu2+	inhibits at high concentrations, stimulates below 0.001 mM	Helicobacter pylori
Zn2+	the enzyme contains one mol of Zn per monomer, apoenzyme is catalytically inactive	Helicobacter pylori

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-
Helicobacter pylori	-	J99	-

Purification (Commentary)

Purification (Comment)	Organism
-	Helicobacter pylori

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
phosphoenolpyruvate + D-arabinose 5-phosphate	-	Escherichia coli	2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate	-	?
phosphoenolpyruvate + D-arabinose 5-phosphate	-	Helicobacter pylori	2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.49	-	phosphoenolpyruvate	pH 6.5, 25°C, Zn2+-enzyme	Helicobacter pylori
0.49	-	D-arabinose 5-phosphate	pH 6.5, 25°C, Zn2+-enzyme	Helicobacter pylori
1.08	-	phosphoenolpyruvate	pH 6.5, 25°C, Cd2+-enzyme	Helicobacter pylori
1.08	-	D-arabinose 5-phosphate	pH 6.5, 25°C, Cd2+-enzyme	Helicobacter pylori
5.31	-	phosphoenolpyruvate	pH 6.5, 25°C	Escherichia coli
5.31	-	D-arabinose 5-phosphate	pH 6.5, 25°C	Escherichia coli

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.0293	-	IC50: 0.0293 mM	Helicobacter pylori	1,10-phenanthroline
0.0422	-	IC50: 0.0422 mM	Helicobacter pylori	2,6-pyridine dicarboxylic acid
0.212	-	IC50: 0.212 mM	Helicobacter pylori	EDTA

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Release 2023.1 (January 2023)