Literature summary for 2.5.1.54 extracted from

Zhang, C.; Kang, Z.; Zhang, J.; Du, G.; Chen, J.; Yu, X.
Construction and application of novel feedback-resistant 3-deoxy-D-arabino-heptulosonate-7-phosphate synthases by engineering the N-terminal domain for L-phenylalanine synthesis (2014), FEMS Microbiol. Lett., 353, 11-18.

Search for more literature for 2.5.1.54

Cloned(Commentary)

Cloned (Comment)	Organism
gene aroF, cloning of mutant enzymes in Escherichia coli strain JM109, recombinant overexpression of mutant enzyme variants AroFD(1-9), AroFD(1-10), AroFD(1-12) and AroFD(1-11)	Corynebacterium glutamicum

Protein Variants

Protein Variants	Comment	Organism
additional information	random mutagenesis, the AroF variant with a deficiency in residue Ile11 is insensitive to L-tyrosine. Construction of feedback-resistant 3-deoxy-D-arabino-heptulosonate-7-phosphate synthases by engineering the N-terminal domain for L-phenylalanine synthesis, generation of nine AroF variants with truncation of different N-terminal fragments, the variants AroFD(1-9), AroFD(1-10), AroFD(1-12) and, in particular, AroFD(1-11) significantly lead to the accumulation of L-phenylalanine, overview. Mutant AroFDELTA(1-11) does not show any feedback inhibition by L-Phe, demonstrated in a strain that has a high L-Phe level through coexpression of L-Phe producing chorismate mutase-4-prephenate dehydratase	Corynebacterium glutamicum

Inhibitors

Inhibitors	Comment	Organism	Structure
L-tyrosine	feedback inhibition of isozyme AroF	Corynebacterium glutamicum

Organism

Organism	UniProt	Comment	Textmining
Corynebacterium glutamicum	P35170	gene aroF	-
Corynebacterium glutamicum ATCC 13032	P35170	gene aroF	-

Synonyms

Synonyms	Comment	Organism
3-deoxy-D-arabino-heptulosonate-7-phosphate synthase	-	Corynebacterium glutamicum
aroF	-	Corynebacterium glutamicum
DAHP synthase	-	Corynebacterium glutamicum

General Information

General Information	Comment	Organism
metabolism	first enzyme of the shikimate pathway	Corynebacterium glutamicum

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)