Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme uses divalent metal ion to activate a phosphorylated carbohydrate-like substrate | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(2R)-2-(phosphonooxy)propanoic acid | mimicking phosphohemiketal 2 (instable), competitive to substrate phosphoenolpyruvate | Escherichia coli | |
(2S)-2-(phosphonooxy)propanoic acid | mimicking phosphohemiketal 2 (instable), competitive to substrate phosphoenolpyruvate | Escherichia coli | |
(2Z)-3-phosphono-2-(trifluoromethyl)prop-2-enoic acid | trifluorinated phosphonate | Escherichia coli | |
(E)-2-methyl-3-phosphonoacrylic acid | most potent of the tested inhibitors mimicking intermediates in the reaction, vinyl phosphonate 4 | Escherichia coli | |
2-(phosphonomethyl)prop-2-enoic acid | mimics substrate phosphoenolpyruvate, and should be inert due to alkene structure but lacking an electron-donor, acts as competitive inhibitor | Escherichia coli | |
fosmidomycin | uncompetitive inhibitor, maximum level of inhibition after 10 min incubation, extent of inhibition dependent on the type of the metal cofactor, competitive inhibitor with respect to phosphoenolpyruvate | Escherichia coli | |
additional information | structure and reaction intermediate mimic inhibitors; sulfoenolpyruvate 7 mimics substrate phosphoenolpyruvate, sulfate exchanges phosphate ester, no inhibition up to a concentration of 10 mM | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 2fold lower inhibition by fosmidomycin with Co2+ as cofactor than with Mn2+ or Fe2+, no effect with sulfoenolpyruvate 7 as potential inhibitor | Escherichia coli | |
Fe2+ | 2fold greater inhibition by fosmidomycin with Fe2+ as cofactor than with Co2+, no effect with sulfoenolpyruvate 7 as potential inhibitor | Escherichia coli | |
Mn2+ | 2fold greater inhibition by fosmidomycin with Mn2+ as cofactor than with Co2+, no effect with sulfoenolpyruvate 7 as potential inhibitor | Escherichia coli | |
Zn2+ | no effect with sulfoenolpyruvate 7 as potential inhibitor | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | Escherichia coli | - |
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Escherichia coli | 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-deoxy-D-arabino-heptulosonate 7-phosphate synthase | - |
Escherichia coli |
DAH7P | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0047 | - |
(E)-2-methyl-3-phosphonoacrylic acid | buffer bis-tris-propane, 50 mM, pH 6.8, Mn2+, UV-visible spectroscopy at 232 nm, substrates phosphoenolpyruvate and D-erythrose 4-phosphate | Escherichia coli | |
0.0088 | - |
(2Z)-3-phosphono-2-(trifluoromethyl)prop-2-enoic acid | buffer bis-tris-propane, 50 mM, pH 6.8, Mn2+, UV-visible spectroscopy at 232 nm, substrates phosphoenolpyruvate and D-erythrose 4-phosphate | Escherichia coli | |
0.035 | - |
fosmidomycin | buffer bis-tris-propane, 50 mM, pH 6.8, Mn2+, UV-visible spectroscopy at 232 nm, substrates phosphoenolpyruvate and D-erythrose 4-phosphate | Escherichia coli | |
0.049 | - |
(2R)-2-(phosphonooxy)propanoic acid | buffer bis-tris-propane, 50 mM, pH 6.8, Mn2+, UV-visible spectroscopy at 232 nm, substrates phosphoenolpyruvate and D-erythrose 4-phosphate | Escherichia coli | |
0.27 | - |
2-(phosphonomethyl)prop-2-enoic acid | buffer bis-tris-propane, 50 mM, pH 6.8, Mn2+, UV-visible spectroscopy at 232 nm, substrates phosphoenolpyruvate and D-erythrose 4-phosphate | Escherichia coli | |
0.67 | - |
(2S)-2-(phosphonooxy)propanoic acid | buffer bis-tris-propane, 50 mM, pH 6.8, Mn2+, UV-visible spectroscopy at 232 nm, substrates phosphoenolpyruvate and D-erythrose 4-phosphate | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
metabolism | catalysis of first step of shipmate pathway to aromatic amino acids | Escherichia coli |