Cloned (Comment) | Organism |
---|---|
- |
Thermotoga maritima |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | inactivation, reactivation in decreasing order by Zn2+, Cd2+, Mn2+, Cu2+, Co2+, Ni2+ | Thermotoga maritima | |
L-phenylalanine | 1 mM, 32% residual activity | Thermotoga maritima | |
L-tyrosine | 1 mM, 23% residual activity | Thermotoga maritima | |
additional information | not inhibitory: L-tryptophan, chorismate, shikimate, D-phenylalanine, L-histidine | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0095 | - |
phosphoenolpyruvate | pH 7.5, 70°C | Thermotoga maritima | |
0.0117 | - |
phosphoenolpyruvate | pH 7.5, 60°C | Thermotoga maritima | |
0.013 | - |
phosphoenolpyruvate | pH 7.5, 50°C | Thermotoga maritima | |
0.0573 | - |
D-erythrose 4-phosphate | pH 7.5, 50°C | Thermotoga maritima | |
0.138 | - |
D-erythrose 4-phosphate | pH 7.5, 60°C | Thermotoga maritima | |
0.3506 | - |
D-erythrose 4-phosphate | pH 7.5, 70°C | Thermotoga maritima |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | reactivation of EDTA-inactivated enzyme in decreasing order by Zn2+, Cd2+, Mn2+, Cu2+, Co2+, Ni2+ | Thermotoga maritima | |
Zn2+ | - |
Thermotoga maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
4 * 38000, SDS-PAGE | Thermotoga maritima |
134000 | - |
gel filtration | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
recombinant enzyme | - |
Renatured (Comment) | Organism |
---|---|
reactivation of EDTA-inactivated enzyme in decreasing order by Zn2+, Cd2+, Mn2+, Cu2+, Co2+, Ni2+ | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Thermotoga maritima | 3-deoxy-D-erythro-hept-2-ulosonate 7-phosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 38000, SDS-PAGE | Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
- |
Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
86h, 50% residual activity | Thermotoga maritima |
70 | - |
21h, 50% residual activity | Thermotoga maritima |
80 | - |
5h, 50% residual activity | Thermotoga maritima |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.3 | - |
phosphoenolpyruvate | pH 7.5, 50°C | Thermotoga maritima | |
5 | - |
phosphoenolpyruvate | pH 7.5, 60°C | Thermotoga maritima | |
7.6 | - |
phosphoenolpyruvate | pH 7.5, 70°C | Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.3 | - |
60°C | Thermotoga maritima |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | more than 90% of maximum activity | Thermotoga maritima |