Literature summary for 2.5.1.54 extracted from

Xu, J.; Hu, C.; Shen, S.; Wang, W.; Jiang, P.; Huang, W.
Requirement of the N-terminus for dimer formation of phenylalanine-sensitive 3-deoxy-D-arabino-heptulosonate synthase AroG of Escherichia coli (2004), J. Basic Microbiol., 44, 400-406.

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Protein Variants

Protein Variants	Comment	Organism
DELTA1-15	N-terminal deletion of amino acids 1-15, no formation of dimeric form	Escherichia coli
I10A	kinetic parameter similar to wild-type, part of enzyme is monomer instead of dimer	Escherichia coli
N5K	kinetic parameter similar to wild-type	Escherichia coli

General Stability

General Stability	Organism
37°C, 65 min, 50% residual activity	Escherichia coli

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.028	-	phosphoenolpyruvate	mutant N5K, 25°C, pH 6.4	Escherichia coli
0.031	-	phosphoenolpyruvate	mutant I10A, 25°C, pH 6.4	Escherichia coli
0.035	-	phosphoenolpyruvate	wild-type, 25°C, pH 6.4	Escherichia coli
0.25	-	D-erythrose 4-phosphate	wild-type, 25°C, pH 6.4	Escherichia coli
0.278	-	D-erythrose 4-phosphate	mutant I10A, 25°C, pH 6.4	Escherichia coli
0.392	-	D-erythrose 4-phosphate	mutant N5K, 25°C, pH 6.4	Escherichia coli
12.25	-	phosphoenolpyruvate	N-terminal deletion mutant, 25°C, pH 6.4	Escherichia coli
52.36	-	D-erythrose 4-phosphate	N-terminal deletion mutant, 25°C, pH 6.4	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	isoform AroG	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O	-	Escherichia coli	3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate	-	?

Subunits

Subunits	Comment	Organism
dimer	wild-type, SDS-PAGE	Escherichia coli

Synonyms

Synonyms	Comment	Organism
AroG	-	Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
4.2	-	phosphoenolpyruvate	N-terminal deletion mutant, 25°C, pH 6.4	Escherichia coli
56.7	-	phosphoenolpyruvate	mutant N5K, 25°C, pH 6.4	Escherichia coli
58.2	-	phosphoenolpyruvate	mutant I10A, 25°C, pH 6.4	Escherichia coli
62.3	-	phosphoenolpyruvate	wild-type, 25°C, pH 6.4	Escherichia coli

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Release 2023.1 (January 2023)