Cloned (Comment) | Organism |
---|---|
Phe-sensitive isozyme, wild-type and mutants, expression in Escherichia coli strain BL21(DE3) | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
C61G | site-directed mutagenesis, highly reduced activity, highly increased Km for phosphoenolpyruvate, higher pH-optimum than the wild-type | Escherichia coli |
H172G | site-directed mutagenesis, inactive | Escherichia coli |
H207G | site-directed mutagenesis, reduced activity, increased Km values for the substrates, reduced kcat | Escherichia coli |
H268G | site-directed mutagenesis, inactive | Escherichia coli |
H304G | site-directed mutagenesis, reduced activity, increased Km values for the substrates, increased kcat | Escherichia coli |
H64G | site-directed mutagenesis, reduced activity, increased Km values for the substrates, increased kcat | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diethyl dicarbonate | Phe-sensitive isozyme, pH-dependent, phosphoenolpyruvate protects wild-type and mutants H64G, H207G, H304G | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
phosphoenolpyruvate | Phe-sensitive isozyme, pH 6.8, 25°C | Escherichia coli | |
0.086 | - |
D-erythrose 4-phosphate | Phe-sensitive isozyme, pH 6.8, 25°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
phenylalanine-sensitive isozyme | - |
Purification (Comment) | Organism |
---|---|
Phe-sensitive isozyme, recombinant wild-type from overexpressing strain and recombinant mutants | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate | His268 is located in the active site | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Escherichia coli | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
ir |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
32 | - |
phosphoenolpyruvate | Phe-sensitive isozyme, pH 6.8, 25°C | Escherichia coli | |
32 | - |
D-erythrose 4-phosphate | Phe-sensitive isozyme, pH 6.8, 25°C | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
wild-type Phe-sensitive isozyme | Escherichia coli |
8 | - |
above, Phe-sensitive iaozyme mutant C61G | Escherichia coli |