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Literature summary for 2.5.1.54 extracted from

  • Whitaker, R.J.; Fiske, M.J.; Jensen, R.A.
    Pseudomonas aeruginosa possesses two novel regulatory isozymes of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (1982), J. Biol. Chem., 257, 12789-12794.
    View publication on PubMed
Search for more literature for 2.5.1.54

Inhibitors

Inhibitors Comment Organism Structure
chorismate competitive against phosphoenolpyruvate, noncompetitive against D-erythrose 4-phosphate; DAHP synthase-trp Pseudomonas aeruginosa
phenylpyruvate DAHP synthase-tyr Pseudomonas aeruginosa
Trp DAHP synthase-trp; non-competitive against phosphoenolpyruvate and competitive for D-erythrose 4-phosphate; Trp-sensitive isozyme Pseudomonas aeruginosa
Tyr DAHP synthase-tyr; L-Tyr; Tyr-sensitive isozyme Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.56
-
 D-erythrose 4-phosphate DAHP synthase-trp, pH 7.0, 37°C Pseudomonas aeruginosa
1
-
 phosphoenolpyruvate DAHP synthase-tyr, pH 7.0, 37°C Pseudomonas aeruginosa
1
-
 D-erythrose 4-phosphate DAHP synthase-tyr, pH 7.0, 37°C Pseudomonas aeruginosa
1.11
-
 phosphoenolpyruvate DAHP synthase-trp, pH 7.0, 37°C Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
137000
-
 DAHP synthase-tyr, gel filtration Pseudomonas aeruginosa
175000
-
 DAHP synthase-trp, gel filtration Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
 2 isoenzymes: tyrosine-sensitive DAHP synthase-tyr and tryptophane-sensitive DAHP synthase-trp
-

Purification (Commentary)

Purification (Comment) Organism
partially, 2 isozymes Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.005
-
 partially purified isozyme synthase-trp Pseudomonas aeruginosa
0.056
-
 partially purified isozyme synthase-tyr Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Pseudomonas aeruginosa 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
 DAHP synthase-tyr Pseudomonas aeruginosa
7
-
 assay at Pseudomonas aeruginosa
7.2
-
 DAHP synthase-trp Pseudomonas aeruginosa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.005
-
 Trp synthase-trp isozyme, versus D-erythrose 4-phosphate, 37°C, pH 7.0 Pseudomonas aeruginosa
0.023
-
 Tyr synthase-tyr isozyme, versus D-erythrose 4-phosphate, 37°C, pH 7.0 Pseudomonas aeruginosa
0.023
-
 Tyr synthase-tyr isozyme, versus phosphoenolpyruvate, 37°C, pH 7.0 Pseudomonas aeruginosa
0.04
-
 Trp synthase-trp isozyme, versus phosphoenolpyruvate, 37°C, pH 7.0 Pseudomonas aeruginosa
1.35
-
 chorismate synthase-trp isozyme, versus phosphoenolpyruvate, 37°C, pH 7.0 Pseudomonas aeruginosa
1.35
-
 phenylpyruvate synthase-tyr isozyme, versus D-erythrose 4-phosphate, 37°C, pH 7.0 Pseudomonas aeruginosa
2.25
-
 chorismate synthase-trp isozyme, versus D-erythrose 4-phosphate, 37°C, pH 7.0 Pseudomonas aeruginosa
2.55
-
 phenylpyruvate synthase-tyr isozyme, versus phosphoenolpyruvate, 37°C, pH 7.0 Pseudomonas aeruginosa