Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-methionine | product inhibition | Clostridioides difficile | |
L-propargylglycine | irreversible, rapid inactivation; suicide inhibitor | Clostridioides difficile | |
Ngamma-acetyl-L-2,4-diaminobutyric acid | competitive; competitive and reversible inhibition | Clostridioides difficile |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
hydrogen sulfide | pH 7.5, 25°C | Clostridioides difficile | |
0.4 | - |
methanethiol | pH 7.5, 25°C | Clostridioides difficile | |
0.6 | - |
O-acetyl-L-homoserine | pH 7.5, 25°C | Clostridioides difficile |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
180000 | - |
gel filtration | Clostridioides difficile |
180000 | - |
gel fitration | Clostridioides difficile |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridioides difficile | Q187D4 | - |
- |
Clostridioides difficile 630 | Q187D4 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
50 | - |
pH 7.5, 25°C | Clostridioides difficile |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-acetyl-L-homoserine + hydrogen sulfide | - |
Clostridioides difficile | L-homocysteine + acetate | - |
? | |
O-acetyl-L-homoserine + hydrogen sulfide | - |
Clostridioides difficile 630 | L-homocysteine + acetate | - |
? | |
O-acetyl-L-homoserine + methanethiol | - |
Clostridioides difficile | L-methionine + acetate | - |
? | |
O-acetyl-L-homoserine + methanethiol | - |
Clostridioides difficile 630 | L-methionine + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 46000, SDS-PAGE, 4 * 46355, calculated from sequence | Clostridioides difficile |
Synonyms | Comment | Organism |
---|---|---|
MetY | - |
Clostridioides difficile |
OAHS | - |
Clostridioides difficile |
Q187D4 | - |
Clostridioides difficile |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
94.12 | - |
O-acetyl-L-homoserine | pH 7.5, 25°C | Clostridioides difficile | |
96.65 | - |
hydrogen sulfide | pH 7.5, 25°C | Clostridioides difficile | |
96.65 | - |
methanethiol | pH 7.5, 25°C | Clostridioides difficile |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
higher activity is shown in potassium phosphate buffer compared to sodium phosphate and Tris-HCl buffers | Clostridioides difficile |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Clostridioides difficile | |
pyridoxal 5'-phosphate | the absorption spectrum of the holoenzyme at pH 7.5 shows a major band in the region of 415-416 nm corresponding to ketoenamine tautomer of the internal aldimines | Clostridioides difficile |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
Ngamma-acetyl-L-2,4-diaminobutyric acid | pH 7.5, 25°C | Clostridioides difficile | |
9 | - |
L-methionine | pH 7.5, 25°C | Clostridioides difficile |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
160 | - |
O-acetyl-L-homoserine | pH 7.5, 25°C | Clostridioides difficile | |
240 | - |
hydrogen sulfide | pH 7.5, 25°C | Clostridioides difficile | |
240 | - |
methanethiol | pH 7.5, 25°C | Clostridioides difficile |