Activating Compound | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Schizosaccharomyces pombe |
General Stability | Organism |
---|---|
very unstable, can be stabilized by addition of 25% sucrose or glycerol | Schizosaccharomyces pombe |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DL-Ethionine | - |
Schizosaccharomyces pombe | |
Hydroxylamine hydrochloride | - |
Schizosaccharomyces pombe | |
KCN | - |
Schizosaccharomyces pombe | |
L-methionine | competitive inhibition | Schizosaccharomyces pombe | |
L-Penicillamine | - |
Schizosaccharomyces pombe | |
additional information | p-chloromercuribenzoic acid, 5,5'-dithio-bis(2-nitrobenzoic acid) and monoiodoacetic acid have no inhibitory effect | Schizosaccharomyces pombe | |
O-acetyl-L-serine | - |
Schizosaccharomyces pombe | |
O-phospho-L-serine | - |
Schizosaccharomyces pombe | |
phenylhydrazine | - |
Schizosaccharomyces pombe | |
S-adenosyl-L-homocysteine | - |
Schizosaccharomyces pombe | |
S-adenosyl-L-methionine | - |
Schizosaccharomyces pombe | |
Semicarbazide | - |
Schizosaccharomyces pombe |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.053 | - |
H2S | - |
Schizosaccharomyces pombe | |
10.4 | - |
L-homoserine | - |
Schizosaccharomyces pombe | |
11.1 | - |
O-succinyl-L-homoserine | - |
Schizosaccharomyces pombe | |
12.5 | - |
O-acetyl-L-homoserine | - |
Schizosaccharomyces pombe |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
170000 | - |
sucrose density gradient ultracentrifugation | Schizosaccharomyces pombe |
186000 | - |
gel filtration | Schizosaccharomyces pombe |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-acetyl-L-homoserine + H2S | Schizosaccharomyces pombe | - |
L-homocysteine + acetic acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizosaccharomyces pombe | - |
fission yeast, IFO-0363 | - |
Purification (Comment) | Organism |
---|---|
- |
Schizosaccharomyces pombe |
Storage Stability | Organism |
---|---|
-20°C, can be stored without loss of catalytic activity | Schizosaccharomyces pombe |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-homoserine + H2S | - |
Schizosaccharomyces pombe | homocysteine + ? | - |
? | |
additional information | does not synthesize cystathionine from O-acetyl-L-homoserine and L-cysteine, cannot utilize cysteine as co-substrate in place of H2S, O-acetyl-L-serine, O-acetyl-L-tyrosine, O-acetyl-L-threonine, O-phospho-DL-threonine, O-phospho-L-serine, L-serine-O-sulfate and O-acetyl-L-hydroxyproline are no substrates | Schizosaccharomyces pombe | ? | - |
? | |
O-acetyl-L-homoserine + H2S | - |
Schizosaccharomyces pombe | L-homocysteine + acetic acid | - |
? | |
O-acetyl-L-serine + H2S | - |
Schizosaccharomyces pombe | L-cysteine + acetic acid | - |
? | |
O-succinyl-L-homoserine + H2S | - |
Schizosaccharomyces pombe | homocysteine + ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Homocysteine synthase | - |
Schizosaccharomyces pombe |
OAH sulfhydrylase | - |
Schizosaccharomyces pombe |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
more stable at 30°C than at 0°C | Schizosaccharomyces pombe |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Schizosaccharomyces pombe |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
optimal pH for stability | Schizosaccharomyces pombe |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Schizosaccharomyces pombe |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
2.6 | - |
L-methionine | - |
Schizosaccharomyces pombe |