Literature summary for 2.5.1.47 extracted from

Singh, K.; Singh, K.P.; Equbal, A.; Suman, S.S.; Zaidi, A.; Garg, G.; Pandey, K.; Das, P.; Ali, V.
Interaction between cysteine synthase and serine O-acetyltransferase proteins and their stage specific expression in Leishmania donovani (2016), Biochimie, 131, 29-44 .

Search for more literature for 2.5.1.47

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Leishmania donovani

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.17	-	H2S	pH 7.5, 37°C	Leishmania donovani
15.86	-	O-acetyl-L-Ser	pH 7.5, 37°C	Leishmania donovani

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	-	Leishmania donovani	5737	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
72000	-	gel filtration	Leishmania donovani

Organism

Organism	UniProt	Comment	Textmining
Leishmania donovani	G1C2I2	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein	Leishmania donovani

Source Tissue

Source Tissue	Comment	Organism	Textmining
promastigote	-	Leishmania donovani	-

Storage Stability

Storage Stability	Organism
-80°C, 10% glycerol, stable for more than 3 months	Leishmania donovani
4°C, 10-15% glycerol, 24 h, no loss of activity	Leishmania donovani

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
O-acetyl-L-Ser + H2S	-	Leishmania donovani	L-Cys + acetate	-	?

Subunits

Subunits	Comment	Organism
dimer	x * 34500, calculated from sequence, x * 38000, SDS-PAGE of His-tagged recombinant protein	Leishmania donovani
More	serine O-acetyltransferase and cysteine synthase form stable complexes containing two serine O-acetyltransferase trimers and two cysteine synthase dimers	Leishmania donovani

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	Leishmania donovani

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	at about 1:1 stoichiometric ratio	Leishmania donovani

pI Value

Organism	Comment	pI Value Maximum	pI Value
Leishmania donovani	calculated from sequence	-	8.2

Expression

Organism	Comment	Expression
Leishmania donovani	serine O-acetyltransferase and cysteine synthase proteins expression/activity is upregulated in amastigote growth stage	up

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Release 2023.1 (January 2023)