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Literature summary for 2.5.1.47 extracted from
- Structural and kinetic insight into the biosynthesis of H2S and L-lanthionine from L-cysteine by a pyridoxal L-phosphate-dependent enzyme from Fusobacterium nucleatum (2019), Biochemistry, 58, 3592-3603 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Fusobacterium nucleatum subsp. nucleatum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.16 | - |
H2S | at pH 7.4 and 25°C | Fusobacterium nucleatum subsp. nucleatum |  |
| 0.41 | - |
O-acetyl-L-serine | at pH 7.4 and 25°C | Fusobacterium nucleatum subsp. nucleatum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fusobacterium nucleatum subsp. nucleatum | - |
- |
- |
| Fusobacterium nucleatum subsp. nucleatum ATCC 25586 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-cysteine + L-cysteine | preferred reaction | Fusobacterium nucleatum subsp. nucleatum | L-lanthionine + H2S | - |
? | |
| L-cysteine + L-cysteine | preferred reaction | Fusobacterium nucleatum subsp. nucleatum ATCC 25586 | L-lanthionine + H2S | - |
? | |
| O-acetyl-L-serine | - |
Fusobacterium nucleatum subsp. nucleatum | L-cysteine + acetate | - |
ir | |
| O-acetyl-L-serine | - |
Fusobacterium nucleatum subsp. nucleatum ATCC 25586 | L-cysteine + acetate | - |
ir | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Fn1220 | - |
Fusobacterium nucleatum subsp. nucleatum |
| lanthionine synthase | - |
Fusobacterium nucleatum subsp. nucleatum |
| OASS | - |
Fusobacterium nucleatum subsp. nucleatum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.3 | - |
O-acetyl-L-serine | at pH 7.4 and 25°C | Fusobacterium nucleatum subsp. nucleatum | |
| 6.3 | - |
H2S | at pH 7.4 and 25°C | Fusobacterium nucleatum subsp. nucleatum | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | dependent on | Fusobacterium nucleatum subsp. nucleatum | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 15 | - |
O-acetyl-L-serine | at pH 7.4 and 25°C | Fusobacterium nucleatum subsp. nucleatum | |
| 40 | - |
H2S | at pH 7.4 and 25°C | Fusobacterium nucleatum subsp. nucleatum | |
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Release 2023.1 (January 2023)
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