Cloned (Comment) | Organism |
---|---|
recombinant expression of chloroplast isozyme OASTL in Arabidopsis thaliana transgenic plants. The cytosolic AtSAT5 isoform from Arabidopsis thaliana is N-terminaly fused with a His-tag, expressed in Escherichia coli and immobilized on a His-trap column for use as affinity anchor to purify enzyme OASTL | Chlorella sorokiniana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Chlorella sorokiniana | 9507 | - |
cytosol | - |
Chlorella sorokiniana | 5829 | - |
additional information | in the unicellular green alga Chlorella sorokiniana two OASTL isoforms, chloroplastic and cytosolic, occur among which the cytosolic is induced under S-deprivation, uniquely altered amounts and activities of OASTL during S-starvation as opposed to the vascular plant systems, e.g. a 17fold increase in the specific activity of enzyme OASTL | Chlorella sorokiniana | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31900 | - |
- |
Chlorella sorokiniana |
33800 | - |
- |
Chlorella sorokiniana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-acetyl-L-serine + hydrogen sulfide | Chlorella sorokiniana | - |
L-cysteine + acetate | - |
? | |
O-acetyl-L-serine + hydrogen sulfide | Chlorella sorokiniana 211/8 K | - |
L-cysteine + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlorella sorokiniana | - |
- |
- |
Chlorella sorokiniana 211/8 K | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant chloroplast isozyme OASTL 29fold from Chlorella sorokiniana S-sufficient and S-starved cells by affinity binding to Arabidopsis thaliana serine acetyltransferase SAT5 fused to a metal resin via its His-tag protein, elution by by O-acetylserine, to homogeneity. Successful application of SAT/OASTL interaction for purification | Chlorella sorokiniana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
149 | - |
purified recombinant enzyme, pH 7.5, temperature not specified in the publication | Chlorella sorokiniana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-acetyl-L-serine + hydrogen sulfide | - |
Chlorella sorokiniana | L-cysteine + acetate | - |
? | |
O-acetyl-L-serine + hydrogen sulfide | - |
Chlorella sorokiniana 211/8 K | L-cysteine + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 33800, chloroplast isozyme OASTL, SDS-PAGE, x * 31900, cytosolic isozyme OASTL, SDS-PAGE | Chlorella sorokiniana |
Synonyms | Comment | Organism |
---|---|---|
O-acetylserine(thiol)lyase | - |
Chlorella sorokiniana |
OASTL | - |
Chlorella sorokiniana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Chlorella sorokiniana |
Organism | Comment | Expression |
---|---|---|
Chlorella sorokiniana | S-starvation results in the increase of the larger chloroplastic OASTL isoform and the induction of a new and smaller cytosolic isoform | up |
General Information | Comment | Organism |
---|---|---|
metabolism | key enzyme in the biosynthetic cysteine pathway | Chlorella sorokiniana |
physiological function | occurrence of the regulatory cysteine synthase complex in microalgae. In plants and also in bacteria, enzyme OASTL is catalytically inactive in the cysteine synthase complex but becomes fully active upon dissociation from the complex realized by O-acetylserine | Chlorella sorokiniana |