Cloned (Comment) | Organism |
---|---|
expressed as a GST-fusion protein | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
F143A | mutant retains one molecule of pyridoxal 5'-phosphate per subunit, mutant reacts with O-acetylserine but the rate is significantly smaller, kcat/KM (O-acetylserine): 950/Msec | Escherichia coli |
F143D | mutant retains one molecule of pyridoxal 5'-phosphate per subunit, mutant reacts with O-acetylserine but the rate is significantly smaller, kcat/KM (O-acetylserine): 150/Msec | Escherichia coli |
F143S | mutant retains one molecule of pyridoxal 5'-phosphate per subunit, mutant reacts with O-acetylserine but the rate is significantly smaller, kcat/KM (O-acetylserine): 380/Msec | Escherichia coli |
F143Y | mutant retains one molecule of pyridoxal 5'-phosphate per subunit, reaction with O-acetylserine is inhibited | Escherichia coli |
Q142A | ability of pyridoxal 5'-phosphate binding is not altered, mutant does not react with O-acetylserine | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using a GSTrap 4 B column and anion-exchange chromatography | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
O-acetylserine sulfhydrylase | - |
Escherichia coli |
OASS | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
O-acetyl-L-serine | mutant F143D | Escherichia coli | |
0.38 | - |
O-acetyl-L-serine | mutant F143S | Escherichia coli | |
0.95 | - |
O-acetyl-L-serine | mutant F143A | Escherichia coli | |
15 | - |
O-acetyl-L-serine | wild-type | Escherichia coli |