Protein Variants | Comment | Organism |
---|---|---|
W161Y | 2fold increase in Vmax and Km-value of O3-acetyl-L-serine | Salmonella enterica subsp. enterica serovar Typhimurium |
W50Y | no effect on catalytic rate or affinity of enzyme to first substrate, Km-value for 5-thio-2-nitrobenzoate decrease by 2.7fold | Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
5-thio-2-nitrobenzoate | mutant W50Y, pH 7.0, 20°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.049 | - |
5-thio-2-nitrobenzoate | wild-type, pH 7.0, 20°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.061 | - |
5-thio-2-nitrobenzoate | mutant W161Y, pH 7.0, 20°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.749 | - |
O3-acetyl-L-serine | wild-type, pH 7.0, 20°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.774 | - |
O3-acetyl-L-serine | mutant W50Y, pH 7.0, 20°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
1.771 | - |
O3-acetyl-L-serine | mutant W161Y, pH 7.0, 20°C | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
isoform OASS-A | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O3-acetyl-L-serine + 2-nitro-5-thiobenzoate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? |