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Literature summary for 2.5.1.47 extracted from
- Structure of the O-acetylserine sulfhydrylase isoenzyme CysM from Escherichia coli (2005), Biochemistry, 44, 8620-8626.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Escherichia coli |
| hanging drop vapor diffusion method, using 100 mM sodium citrate, pH 5.6, 100 mM ammonium sulfate, 20% (w/v) PEG 4000 | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32893 | - |
2 * 32893, calculated from amino acid sequence | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| O-acetyl-L-serine + hydrogen sulfide | Escherichia coli | - |
L-cysteine + acetate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P16703 | - |
- |
| Escherichia coli | P16703 | isoenzyme CysM | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Source 30Q column chromatography and Superdex 75 gel filtration | Escherichia coli |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate | geometric model of reaction, comparison isoenzyme CysK | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| O-acetyl-L-serine + hydrogen sulfide | - |
Escherichia coli | L-cysteine + acetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 32893, calculated from amino acid sequence | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CysM | - |
Escherichia coli |
| O-acetylserine sulfhydrylase isoenzyme B | - |
Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Escherichia coli | |
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