Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) | 1 mM, 92% inactivation | Thermus thermophilus | |
hydroxylamine | 10 mM, 71.2% inhibition | Thermus thermophilus | |
KCN | 15.6% inhibition by 1 mM | Thermus thermophilus | |
Monoiodoacetic acid | 1 mM, complete inactivation | Thermus thermophilus | |
phenylhydrazine | 73% inhibition by 1 mM, 97.4% inhibition by 10 mM | Thermus thermophilus | |
Semicarbazide | 13% inhibition by 1 mM, 38.6% inhibition by 10 mM | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
H2S | pH 7.8, 50°C | Thermus thermophilus | |
4.8 | - |
O-acetyl-L-Ser | pH 7.8, 50°C | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
2 * 34000, SDS-PAGE | Thermus thermophilus |
68000 | - |
gel filtration | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
HB8 | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
HB8 | - |
Purification (Comment) | Organism |
---|---|
- |
Thermus thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
337.4 | - |
- |
Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chloroalanine + sulfide | beta-chloroalanine, 6% of the activity with O-acetyl-L-Ser | Thermus thermophilus | cysteine + chloride | - |
r | |
chloroalanine + sulfide | beta-chloroalanine, 6% of the activity with O-acetyl-L-Ser | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | cysteine + chloride | - |
r | |
L-homoserine + sulfide | 1.6% of the activity with O-acetyl-L-serine | Thermus thermophilus | ? | - |
? | |
O-acetyl-L-Ser + H2S | - |
Thermus thermophilus | L-Cys + acetate | - |
? | |
O-acetyl-L-Ser + H2S | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | L-Cys + acetate | - |
? | |
O-acetyl-L-serine | - |
Thermus thermophilus | L-cysteine + acetate | - |
? | |
O-acetylhomoserine + H2S | 2.4% of the activity with O-acetyl-L-Ser | Thermus thermophilus | homocysteine + ? | - |
? | |
O-acetylhomoserine + H2S | 2.4% of the activity with O-acetyl-L-Ser | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | homocysteine + ? | - |
? | |
O-diazoacetyl-L-serine + sulfide | 44% of the activity with O-acetyl-Ser | Thermus thermophilus | ? | - |
? | |
O-diazoacetyl-L-serine + sulfide | 44% of the activity with O-acetyl-Ser | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ? | - |
? | |
O-succinyl-L-homoserine + sulfide | 3.6% of the activity with O-acetyl-L-serine | Thermus thermophilus | ? | - |
? | |
O-succinyl-L-homoserine + sulfide | 3.6% of the activity with O-acetyl-L-serine | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ? | - |
? | |
Ser + sulfide | 1.8% of the activity with O-acetyl-L-serine | Thermus thermophilus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 34000, SDS-PAGE | Thermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Thermus thermophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 67 | about 50% of maximal activity at 35°C and at 67°C | Thermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
pH 7.8, 60 min, stable | Thermus thermophilus |
80 | - |
60 min, 10 mM dithiothreitol, less than 10% of activity | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Thermus thermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8 | about 50% of maximal activity at pH 5.5 and pH 8.0 | Thermus thermophilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | 12 | 50°C, 30 min, stable | Thermus thermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | Km of the cofactor at pH 8.0: 0.029 mM | Thermus thermophilus |