General Stability | Organism |
---|---|
chloroplast enzyme very unstable | Spinacia oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
H2S | pH 7.5, 25°C, chloroplast enzyme | Spinacia oleracea | |
1.3 | - |
O-acetyl-L-Ser | pH 7.5, 25°C, chloroplast enzyme | Spinacia oleracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | different from cytosolic and mitochondrial enzymes, as revealed by polyclonal antibody studies, 3-5% of total activity | Spinacia oleracea | 9507 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
2 * 35000, SDS-PAGE | Spinacia oleracea |
68000 | - |
chloroplast enzyme, gel filtration | Spinacia oleracea |
310000 | - |
cysteine synthetase complex, gel filtration | Spinacia oleracea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Spinacia oleracea | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
96 | - |
chloroplast enzyme | Spinacia oleracea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-acetyl-L-serine | - |
Spinacia oleracea | L-cysteine + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 35000, SDS-PAGE | Spinacia oleracea |
More | amino acid composition | Spinacia oleracea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
chloroplast enzyme | Spinacia oleracea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Spinacia oleracea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | 1.1 mol per mol subunit | Spinacia oleracea |