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Literature summary for 2.5.1.47 extracted from

  • Ikegami, F.; Kaneko, M.; Kamiyama, H.; Murakoshi, I.
    Purification and characterization of cysteine synthases from Citrullus vulgaris (1988), Phytochemistry, 27, 697-701.
No PubMed abstract available
Search for more literature for 2.5.1.47

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.033
-
 H2S pH 8.0, 30°C, isoenzyme 1 Citrullus lanatus subsp. vulgaris
1.5
-
 O-acetyl-L-Ser pH 8.0, 30°C, isoenzyme 2 Citrullus lanatus subsp. vulgaris
2.6
-
 O-acetyl-L-Ser pH 8.0, 30°C, isoenzyme 1 Citrullus lanatus subsp. vulgaris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 2 * 29000, SDS-PAGE Citrullus lanatus subsp. vulgaris
58000
-
 gel filtration Citrullus lanatus subsp. vulgaris

Organism

Organism UniProt Comment Textmining
Citrullus lanatus subsp. vulgaris
-
 two isoforms
-

Purification (Commentary)

Purification (Comment) Organism
-
 Citrullus lanatus subsp. vulgaris

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
 Citrullus lanatus subsp. vulgaris
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
649
-
 isoenzyme 1 Citrullus lanatus subsp. vulgaris
691
-
 isoenzyme 2 Citrullus lanatus subsp. vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information beta-substituted alanines, low activity Citrullus lanatus subsp. vulgaris ?
-
 ?
O-acetyl-L-Ser + 2-propene-1-thiol 6.2% of the activity with sulfide, isoenzyme 2 Citrullus lanatus subsp. vulgaris S-allyl-L-cysteine + ?
-
 ?
O-acetyl-L-Ser + H2S
-
 Citrullus lanatus subsp. vulgaris L-Cys + acetate
-
 ?
O-acetyl-L-Ser + mercaptoacetic acid 2.2% of activity with sulfide, isoenzyme 1 and 2 Citrullus lanatus subsp. vulgaris S-carboxymethyl-L-cysteine
-
 ?
O-acetyl-L-Ser + methyl mercaptan 21.5% and 77% of activity with sulfide, isoenzymes 1 and 2, respectively Citrullus lanatus subsp. vulgaris S-methylcysteine + acetate
-
 ?
O-acetyl-L-serine
-
 Citrullus lanatus subsp. vulgaris L-cysteine + acetate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 29000, SDS-PAGE Citrullus lanatus subsp. vulgaris
More amino acid composition Citrullus lanatus subsp. vulgaris
More isoenzymes have different cysteine and methionine content Citrullus lanatus subsp. vulgaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Citrullus lanatus subsp. vulgaris