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Literature summary for 2.5.1.47 extracted from

  • Ikegami, F.; Kaneko, M.; Kobori, M.; Murakoshi, I.
    Purification and characterization of cysteine synthase from Brassica juncea (1988), Phytochemistry, 27, 3379-3383.
No PubMed abstract available
Search for more literature for 2.5.1.47

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.043
-
 H2S pH 8.0, 30°C Brassica juncea
2.5
-
 O-acetyl-L-Ser pH 8.0, 30°C Brassica juncea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 2 * 26000, SDS-PAGE Brassica juncea
52000
-
 gel filtration Brassica juncea

Organism

Organism UniProt Comment Textmining
Brassica juncea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Brassica juncea

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Brassica juncea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
410
-
-
 Brassica juncea

Storage Stability

Storage Stability Organism
0 C, potassium phosphate buffer, pH 8, mercaptoethanol, EDTA, three months, no loss of activity Brassica juncea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information beta-substituted alanines, low activity Brassica juncea ?
-
 ?
O-acetyl-L-Ser + 2-propene-1-thiol 18% of activity with sulfide Brassica juncea S-allyl-L-cysteine + ?
-
 ?
O-acetyl-L-Ser + H2S
-
 Brassica juncea L-Cys + acetate
-
 ?
O-acetyl-L-Ser + mercaptoacetic acid 6.1% of activity with sulfide Brassica juncea S-carboxymethyl-L-cysteine
-
 ?
O-acetyl-L-Ser + NaCN 12.3% of activity with sulfide Brassica juncea beta-cyanoalanine + sodium acetate
-
 ?
O-acetyl-L-serine
-
 Brassica juncea L-cysteine + acetate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 26000, SDS-PAGE Brassica juncea
More amino acid composition Brassica juncea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Brassica juncea

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate one per subunit Brassica juncea