Application | Comment | Organism |
---|---|---|
drug development | Cryptosporidium parvum DHS is a traget for drug development, as GC7 effectively inhibits parasite infection and growth in cultured host cells | Cryptosporidium parvum |
Cloned (Comment) | Organism |
---|---|
gene DHS, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Cryptosporidium parvum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,8-diaminooctane | weaker inhibition | Cryptosporidium parvum | |
diaminononane | weaker inhibition | Cryptosporidium parvum | |
N1-guanyl-1,7-diaminoheptane | GC7, a potent competitive inhibitor of DHS, causes an effective inhibition of infection and growth of Cryptosporidium parvum in human HCT-8 adenocarcinoma cells. Complete inhibition at 0.01 mM | Cryptosporidium parvum | |
paromomycin | inhibits oocysts formation | Cryptosporidium parvum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00091 | - |
[eIF5A-precursor]-lysine | pH 7.5, 37°C, heterotetrameric DHS | Cryptosporidium parvum | |
0.0123 | - |
spermidine | pH 7.5, 37°C, heterotetrameric DHS | Cryptosporidium parvum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40800 | - |
- |
Cryptosporidium parvum |
180000 | - |
native PAGE | Cryptosporidium parvum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[eIF5A-precursor]-lysine + spermidine | Cryptosporidium parvum | - |
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cryptosporidium parvum | A3FQA5 | single-copy gene DHS | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) to homogeneity by nickel affinity chromatography | Cryptosporidium parvum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[eIF5A-precursor]-lysine + spermidine | - |
Cryptosporidium parvum | [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 40800, about, sequence calculation | Cryptosporidium parvum |
Synonyms | Comment | Organism |
---|---|---|
CpDHS | - |
Cryptosporidium parvum |
DHS | - |
Cryptosporidium parvum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Cryptosporidium parvum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.2 | - |
assay at | Cryptosporidium parvum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | required | Cryptosporidium parvum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition profile in a series of kinetic experiments | Cryptosporidium parvum |
General Information | Comment | Organism |
---|---|---|
evolution | CpDHS is more closely related to apicomplexan and human DHS than to kinetoplast DHS | Cryptosporidium parvum |
additional information | enzyme structure modelling, model evaluation and comparative structural analysis, overview | Cryptosporidium parvum |
physiological function | hypusination of eIF5A is an important post-translational modification essential for cell proliferation. This modification occurs in a two step processcatalyzed by deoxyhypusine synthase followed by deoxyhypusine hydroxylase | Cryptosporidium parvum |