Cloned (Comment) | Organism |
---|---|
gene Tb10.70.4900, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21 (DE3), coexpression with gene TB927.1.870 results in heterotetramer formation with a 3000fold increase in enzyme activity | Trypanosoma brucei brucei |
gene TB927.1.870, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21 (DE3), coexpression with gene Tb10.70.4900 in Escherichia coli strain BL21 (DE3) results in heterotetramer formation with a 3000fold increase in enzyme activity | Trypanosoma brucei brucei |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of a knockout mutant by gene replacement | Trypanosoma brucei brucei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N1-guanyl-1,7-diaminoheptane | GC7, inhibits the activity of TbDHSc-TbDBHSp heterotetrameric complex; GC7, inhibits the activity of TbDHSc-TbDBHSp heterotetrameric complex | Trypanosoma brucei brucei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0007 | - |
[eIF5A-precursor]-lysine | pH 7.5, 37°C, heterotetrameric DHS | Trypanosoma brucei brucei | |
0.043 | - |
spermidine | pH 7.5, 37°C, heterotetrameric DHS | Trypanosoma brucei brucei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
kinetoplast | - |
Trypanosoma brucei brucei | 20023 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[eIF5A-precursor]-lysine + spermidine | Trypanosoma brucei brucei | - |
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine | - |
? | |
[eIF5A-precursor]-lysine + spermidine | Trypanosoma brucei brucei 927 / 4 GUTat10.1 / TREU927 | - |
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei brucei | Q38BX0 | gene Tb10.70.4900 | - |
Trypanosoma brucei brucei | Q4GZD1 | gene TB927.1.870 | - |
Trypanosoma brucei brucei 927 / 4 GUTat10.1 / TREU927 | Q38BX0 | gene Tb10.70.4900 | - |
Trypanosoma brucei brucei 927 / 4 GUTat10.1 / TREU927 | Q4GZD1 | gene TB927.1.870 | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Trypanosoma brucei brucei |
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity cromatography and gel filtration | Trypanosoma brucei brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[eIF5A-precursor]-lysine + spermidine | - |
Trypanosoma brucei brucei | [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine | - |
? | |
[eIF5A-precursor]-lysine + spermidine | - |
Trypanosoma brucei brucei 927 / 4 GUTat10.1 / TREU927 | [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DHS | - |
Trypanosoma brucei brucei |
TbDHSc | - |
Trypanosoma brucei brucei |
TbDHSp | - |
Trypanosoma brucei brucei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Trypanosoma brucei brucei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
spermidine | pH 7.5, 37°C, heterotetrameric DHS | Trypanosoma brucei brucei | |
0.018 | - |
[eIF5A-precursor]-lysine | pH 7.5, 37°C, heterotetrameric DHS | Trypanosoma brucei brucei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Trypanosoma brucei brucei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | required, Km value is 0.082 mM, kcat is 0.018/s for the heterotetrameric DHS | Trypanosoma brucei brucei |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0015 | - |
pH 7.5, 37°C, heterotetrameric DHS | Trypanosoma brucei brucei | N1-guanyl-1,7-diaminoheptane |
General Information | Comment | Organism |
---|---|---|
additional information | Trypanosoma brucei encodes two deoxyhypusine synthase isozymes TbDHSc and TbDHSp that form a functional complex | Trypanosoma brucei brucei |
additional information | Trypanosoma brucei encodes two deoxyhypusine synthase isozymes TbDHSc and TbDHSp that form a functional heterotetrameric complex | Trypanosoma brucei brucei |
physiological function | the enzyme deoxyhypusine synthase is required for spermidine-dependent hypusine modification of a lysine residue in the essential translation factor eIF5A. Enzyme DHS is essential for infectivity of Trypanosoma brucei in mice | Trypanosoma brucei brucei |