Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(R)-3-methylspermidine | competitive inhibition versus spermidine | Homo sapiens | |
(S)-3-methylspermidine | competitive inhibition versus spermidine | Homo sapiens | |
additional information | no growth inhibition by alpha-difluoromethylornithine, an inhibitor of polyamine biosynthesis, and (R)-3-methylspermidine, while the the combination of alpha-difluoromethylornithine with a racemate or (S)-3-methylspermidine causes cessation of cell growth. Cells treated with racemic 3-MeSpd accumulat intracellularly mainly (S)-3-methylspermidine, but not DHS substrate (R)-3-methylspermidine, explaining the inability of the racemate to support long-term growth | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[eIF5A-precursor]-lysine + spermidine | Homo sapiens | - |
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P49366 | gene Dhps | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
DU-145 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | (R)-3-methylspermidine competes with spermidine for cellular uptake less efficiently than the (S)-3-methylspermidine | Homo sapiens | ? | - |
? | |
[eIF5A-precursor]-lysine + (R)-3-methylspermidine | enzyme DHS prefers the (R)- over the (S)-enantiomer as a substrate | Homo sapiens | ? | - |
? | |
[eIF5A-precursor]-lysine + spermidine | - |
Homo sapiens | [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine | - |
? | |
[eIF5A-precursor]-lysine + spermidine | formation of [N(epsilon)-(4-amino-2-hydroxybutyl)lysine] | Homo sapiens | [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DHS | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.019 | - |
(S)-3-methylspermidine | pH and temperature not specified in the publication | Homo sapiens | |
0.141 | - |
(R)-3-methylspermidine | pH and temperature not specified in the publication | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | the eukaryotic translation initiation factor 5A (eIF5A) is essential for cell proliferation, becoming functionally active only after post-translational conversion of a specific Lys to hypusine catalyzed by deoxyhypusine synthase, the rate-limiting enzyme of this two-step process | Homo sapiens |