Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[eIF5A-precursor]-lysine + spermidine | Homo sapiens | - |
[eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | development of a radioactive assay method for detection of spermidine incorporation into eIF5A, overview | Homo sapiens | ? | - |
? | |
[eIF5A-precursor]-lysine + spermidine | - |
Homo sapiens | [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine | - |
? | |
[eIF5A-precursor]-lysine + spermidine | DHS catalyzes a NAD-dependent cleavage between N4 and C5 of spermidine and the transfer of the butylamine moiety to the ?-amino group of a specific lysine residue of the eIF5A precursor, eIF5A(Lys), to form the deoxyhypusine residue. The enzyme is modification is strictly specific for one single lysine residue of the cellular protein eukaryotic initiation factor 5A (eIF5A) and does not work on short peptides | Homo sapiens | [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine | 1,3-diaminopropane is released as a byproduct from spermidine cleavage | ? |
Synonyms | Comment | Organism |
---|---|---|
DHS | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.2 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | required | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | deoxyhypusine synthase catalyzes the synthesis of deoxyhypusine [N?-(4-aminobutyl)-lysine], the first step in the post-translational synthesis of an unusual amino acid hypusine [N?-(4-amino-2-hydroxybutyl)-lysine] | Homo sapiens |