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Literature summary for 2.5.1.46 extracted from

  • Ober, D.; Harms, R.; Witte, L.; Hartmann, T.
    Molecular evolution by change of function. Alkaloid-specific homospermidine synthase retains all properties of deoxyhypusine synthase except binding the eIF5A precursor protein (2003), J. Biol. Chem., 278, 12805-12812.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Senecio vernalis
0.0013
-
[eIF5A-precursor]-L-lysine
-
Senecio vernalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
x * 41000, SDS-PAGE Senecio vernalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[eIF5A-precursor]-lysine + spermidine Senecio vernalis posttranslational activation of the initiation factor 5A [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
?

Organism

Organism UniProt Comment Textmining
Senecio vernalis Q9SC14
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Senecio vernalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
homospermidine + putrescine
-
Senecio vernalis homospermidine + ?
-
?
spermidine + putrescine
-
Senecio vernalis homospermidine + ?
-
?
[eIF5A-precursor]-L-lysine + spermidine
-
Senecio vernalis [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
?
[eIF5A-precursor]-lysine + spermidine
-
Senecio vernalis [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine i.e. [eIF5A-precursor]-N6-(4-aminobutyl)lysine ?
[eIF5A-precursor]-lysine + spermidine posttranslational activation of the initiation factor 5A Senecio vernalis [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
?

Subunits

Subunits Comment Organism
? x * 41000, SDS-PAGE Senecio vernalis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0022
-
[eIF5A-precursor]-L-lysine + spermidine Senecio vernalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.3
-
glycine-NaOH buffer Senecio vernalis

pH Range

pH Minimum pH Maximum Comment Organism
8.8 9.9 pH 8.8: about 35% of maximal activity, pH 9.9: about 60% of maximal activity Senecio vernalis