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Literature summary for 2.5.1.46 extracted from
- Biochemical and immunological characterization of deoxyhypusine synthase purified from the yeast Saccharomyces carlsbergensis (1997), J. Biochem., 121, 769-778.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,3-diaminopropane | - |
Saccharomyces cerevisiae |  |
| 1,7-Diaminoheptane | - |
Saccharomyces cerevisiae | |
| 1,8-diaminooctane | - |
Saccharomyces cerevisiae | |
| 1,9-diaminononane | - |
Saccharomyces cerevisiae | |
| Cu2+ | - |
Saccharomyces cerevisiae | |
| FAD | - |
Saccharomyces cerevisiae | |
| Fe2+ | - |
Saccharomyces cerevisiae | |
| Fe3+ | - |
Saccharomyces cerevisiae | |
| FMN | - |
Saccharomyces cerevisiae | |
| Mn2+ | weak | Saccharomyces cerevisiae | |
| NADH | - |
Saccharomyces cerevisiae | |
| spermidine | - |
Saccharomyces cerevisiae | |
| Zn2+ | weak | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
4 * 43000, SDS-PAGE | Saccharomyces cerevisiae |
| 165000 | 172000 | gel filtration sedimentation equilibrium centrifugation | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| [eIF5A-precursor]-lysine + spermidine | - |
Saccharomyces cerevisiae | [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine | i.e. [eIF5A-precursor]-N6-(4-aminobutyl)lysine | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 43000, SDS-PAGE | Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Saccharomyces cerevisiae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 37 | 20°C: about 75% of maximal activity, 37°C: about 25% of maximal activity | Saccharomyces cerevisiae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | - |
1 h, 35% loss of activity, NAD+ protects | Saccharomyces cerevisiae |
| 65 | - |
15 min, almost complete loss of activity, NAD+ protects | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
- |
Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 11 | pH 8.5: about 45% of maximal activity, pH 11.0: about 50% of maximal activity | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Saccharomyces cerevisiae | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0009 | - |
1,7-Diaminoheptane | - |
Saccharomyces cerevisiae | |
| 0.00191 | - |
1,8-diaminooctane | - |
Saccharomyces cerevisiae | |
| 0.00649 | - |
spermidine | - |
Saccharomyces cerevisiae | |
| 0.00752 | - |
1,9-diaminononane | - |
Saccharomyces cerevisiae | |
| 0.0129 | - |
1,3-diaminopropane | - |
Saccharomyces cerevisiae | |
| 0.85 | - |
NADH | - |
Saccharomyces cerevisiae | |
| 1.28 | - |
FMN | - |
Saccharomyces cerevisiae | |
| 2.27 | - |
FAD | - |
Saccharomyces cerevisiae | |
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Release 2023.1 (January 2023)
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