Cloned (Comment) | Organism |
---|---|
gene ZmNAS1;1, located on chromosome 9, genotying and phylogenetic analysis, sequence comparisons, expression analysis | Zea mays |
gene ZmNAS1;2, located on chromosome 9, genotying and phylogenetic analysis, sequence comparisons, expression analysis | Zea mays |
gene ZmNAS2;1, located on chromosome 1, genotying and phylogenetic analysis, sequence comparisons, expression analysis | Zea mays |
gene ZmNAS2;2, located on chromosome 1, genotying and phylogenetic analysis, sequence comparisons, expression analysis | Zea mays |
gene ZmNAS3, located on chromosome 1, genotying and phylogenetic analysis, sequence comparisons, expression analysis | Zea mays |
gene ZmNAS4, located on chromosome 5, genotying and phylogenetic analysis, sequence comparisons, expression analysis | Zea mays |
gene ZmNAS5, located on chromosome 7, genotying and phylogenetic analysis, sequence comparisons, expression analysis | Zea mays |
gene ZmNAS6;1, located on chromosome 9, genotying and phylogenetic analysis, sequence comparisons, expression analysis | Zea mays |
gene ZmNAS6;2, located on chromosome 9, genotying and phylogenetic analysis, sequence comparisons, expression analysis | Zea mays |
recombinant expression of GFP-tagged isozyme OsNAS2 in Oryza sativa roots. OsNAS2-sGFP vesicles move dynamically in the cells. Fe homeostasis is disturbed in the GFP-tagged OsNAS2 plants, and these plants receive Fe-deficiency signals even under Fe-sufficient conditions, this is probably due to to the overproduction of deocxymugineic acid and nicotinamine, which increases the chelating capacity of Fe and disrupts an unknown Fe-sensing mechanism | Oryza sativa |
Protein Variants | Comment | Organism |
---|---|---|
additional information | isozyme OsNAS2 mutated in the YXXphi motif, mutant m6-sGFP, is localized to the vesicles. These vesicles stuck together and are immobile. OsNAS2 mutated in the LL motif, mutant m7-sGFP, does not localize to these vesicles | Oryza sativa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
vesicle | model for OsNAS2-localized vesicles in Oryza sativa | Oryza sativa | 31982 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 S-adenosyl-L-methionine | Zea mays | - |
3 S-methyl-5'-thioadenosine + nicotianamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | Q10MI9 | subsp. indica, gene NAS2 | - |
Zea mays | A0A1D6GLB9 | gene ZmNAS4 | - |
Zea mays | A0A1D6IP14 | gene ZmNAS5 | - |
Zea mays | A0A1D6K0A7 | gene ZmNAS2;2 | - |
Zea mays | A0A1D6K0A8 | gene ZmNAS2;1 or ZmNAS2 | - |
Zea mays | B4FAC0 | gene ZmNAS6;1 | - |
Zea mays | K7VIY6 | gene ZmNAS6;2 | - |
Zea mays | K7WE51 | gene ZmNAS1;2 | - |
Zea mays | Q8LT22 | gene ZmNAS3 | - |
Zea mays | Q8S9C5 | gene ZmNAS1;1 or ZmNAS1 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | modeling of posttranscriptional mRNA degradation and/or posttranslational protein degradation of isozyme OsNAS2 | Oryza sativa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
axillary meristem | - |
Zea mays | - |
epidermis | of roots | Zea mays | - |
leaf | primordia, low expression level | Zea mays | - |
leaf | ZmNAS3 is predominantly accumulated in leaves, expression in young leaves, mainly in the leaf primordia and mesophyll cells in young leaves | Zea mays | - |
mesophyll cell | - |
Zea mays | - |
additional information | isozyme issue expression patterns, overview. No ZmNAS1;1 expression in shoots | Zea mays | - |
additional information | isozyme tissue expression patterns, overview | Zea mays | - |
additional information | isozyme tissue expression patterns, overview. No ZmNAS1;2 expression in shoots | Zea mays | - |
root | - |
Oryza sativa | - |
root | cortex and stele, epidermis | Zea mays | - |
seed | developing | Zea mays | - |
shoot | low expression level, mainly in axillary meristems in shoot apices | Zea mays | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 S-adenosyl-L-methionine | - |
Zea mays | 3 S-methyl-5'-thioadenosine + nicotianamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NA synthase 2 | - |
Oryza sativa |
OsNA S2 | - |
Oryza sativa |
ZmNAS1;1 | - |
Zea mays |
ZmNAS1;2 | - |
Zea mays |
ZmNAS2;1 | - |
Zea mays |
ZmNAS2;2 | - |
Zea mays |
ZmNAS3 | - |
Zea mays |
ZmNAS4 | - |
Zea mays |
ZmNAS5 | - |
Zea mays |
ZmNAS6;1 | - |
Zea mays |
ZmNAS6;2 | - |
Zea mays |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Zea mays |
Organism | Comment | Expression |
---|---|---|
Zea mays | class I ZmNAS genes are induced under Fe deficiency and are suppressed under Fe excessive conditions, while the expression pattern of class II genes are opposite to class I. Expression patterns of ZmNAS genes in response to fluctuating metal status | down |
Zea mays | class I ZmNAS genes are induced under Fe deficiency and are suppressed under Fe excessive conditions, while the expression pattern of class II genes are opposite to class I. Expression patterns of ZmNAS genes in response to fluctuating metal status. Class I genes are suppressed in response to Zn excess and Cu/Mn deficiency | down |
Zea mays | class I ZmNAS genes are induced under Fe deficiency and are suppressed under Fe excessive conditions, while the expression pattern of class II genes are opposite to class I. Expression patterns of ZmNAS genes in response to fluctuating metal status. Class I ZmNAS genes are stimulated under Zn deficiency | up |
Zea mays | class I ZmNAS genes are induced under Fe deficiency and are suppressed under Fe excessive conditions, while the expression pattern of class II genes are opposite to class I. Expression patterns of ZmNAS genes in response to fluctuating metal status. Class II genes are induced under excessive Zn and deficient Cu/Mn conditions | up |
General Information | Comment | Organism |
---|---|---|
evolution | complementary expression patterns of class I and class II ZmNAS genes in response to Fe confirms the classification of this family, ZmNAS1;1 is a class I enzyme | Zea mays |
evolution | complementary expression patterns of class I and class II ZmNAS genes in response to Fe confirms the classification of this family, ZmNAS1;2 is a class I enzyme | Zea mays |
evolution | complementary expression patterns of class I and class II ZmNAS genes in response to Fe confirms the classification of this family, ZmNAS2;1 is a class I enzyme | Zea mays |
evolution | complementary expression patterns of class I and class II ZmNAS genes in response to Fe confirms the classification of this family, ZmNAS2;2 is a class I enzyme | Zea mays |
evolution | complementary expression patterns of class I and class II ZmNAS genes in response to Fe confirms the classification of this family, ZmNAS3 is a class II enzyme | Zea mays |
evolution | complementary expression patterns of class I and class II ZmNAS genes in response to Fe confirms the classification of this family, ZmNAS4 is a class II enzyme | Zea mays |
evolution | complementary expression patterns of class I and class II ZmNAS genes in response to Fe confirms the classification of this family, ZmNAS5 is a class II enzyme | Zea mays |
evolution | complementary expression patterns of class I and class II ZmNAS genes in response to Fe confirms the classification of this family, ZmNAS6;1 is a class I enzyme | Zea mays |
evolution | complementary expression patterns of class I and class II ZmNAS genes in response to Fe confirms the classification of this family, ZmNAS6;2 is a class I enzyme | Zea mays |
malfunction | mutation of the N-terminal tyrosine motif or di-leucine motif of isozyme OsNAS2, involved in cellular transport, causes a disruption in vesicular movement and vesicular localization, respectively. Fe homeostasis is disturbed in the GFP-tagged OsNAS2 plants, and these plants receive Fe-deficiency signals even under Fe-sufficient conditions, this is probably due to to the overproduction of deocxymugineic acid and nicotinamine, which increases the chelating capacity of Fe and disrupts an unknown Fe-sensing mechanism. OsNAS2-sGFP plants grow more slowly than the wild-type and the mutant m6-sGFP and m7-sGFP plants | Oryza sativa |
additional information | the enzyme's tyrosine motif is involved in vesicle movement, whereas the di-leucine motif is involved in vesicle localization and OsNAS2 activity, which are crucial for the proper function of OsNAS2 | Oryza sativa |
physiological function | class I ZmNAS genes may be involved in the Fe uptake in roots and long distance translocation in stems | Zea mays |
physiological function | class II ZmNAS genes may contribute to the local transportation of Fe | Zea mays |
physiological function | class II ZmNAS genes may contribute to the local transportation of Fe. ZmNAS3, a member of class II ZmNAS genes, may participate in the local transportation and homeostasis of Fe in developing tissues | Zea mays |
physiological function | graminaceous plants utilize a chelation strategy to acquire Fe from soil that involves the secretion of mugineic acid family phytosiderophores (MAs), which chelate and solubilize Fe(III) in the rhizosphere from their roots through transporter of mugineic acids 1 (TOM1). The resultant Fe(III)-MAs complexes are absorbed by root cells through a transporter protein YSL. Rice produces and secretes 2'-deoxymugineic acid (DMA). DMA is synthesized from S-adenosylmethionine through a nicotianamine (NA) intermediate8 by 3 enzymes: NA synthase (NAS), NA aminotransferase (NAAT), and DMA synthase. Nicotinamine is a structural analog of mugineic acid, and is responsible for metal homeostasis through metal translocation in plants. Particular vesicles, originating from the rough endoplasmic reticulum, are involved in deoxymugineic acid and nicotianamine biosynthesis and in deoxymugineic acid secretion from Oryza sativa roots. Modeling of the intracellular transport of mugineic acid-vesicles in rice roots | Oryza sativa |