Cloned (Comment) | Organism |
---|---|
gene OsNAS2, recombinant expression of GFP-tagged isozyme NAS2 in transgenic Oryza sativa plants under the control of its own promoter. The recombinant GFP-tagged enzyme moves moving dynamically within root cells, phhenotype, ooverview | Oryza sativa |
Protein Variants | Comment | Organism |
---|---|---|
L115A/L116A | isozyme OsNAS2 mutated in the LL motif resulting in mutant m7-sGFP, which does not localize to the vesicles. Mutant m7sGFP does not show NAS enzyme activity | Oryza sativa |
additional information | mutant enzyme phenotype under both Fe-sufficient and -deficient conditions, overview | Oryza sativa |
Y105A | isozyme OsNAS2 mutated in the YXXphi motif resulting in mutant m6-sGFP, which is localized to the vesicles. These vesicles stuck together and are immobile. Mutant m6-sGFP converts S-adenosyl methionine into nicotinamine in vitro | Oryza sativa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
vesicle | - |
Oryza sativa | 31982 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 S-adenosyl-L-methionine | Oryza sativa | - |
3 S-methyl-5'-thioadenosine + nicotianamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | Q10MI9 | gene OsNAS2 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Oryza sativa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 S-adenosyl-L-methionine | - |
Oryza sativa | 3 S-methyl-5'-thioadenosine + nicotianamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NA synthase 2 | - |
Oryza sativa |
NAS2 | - |
Oryza sativa |
nicotianamine synthase 2 | - |
Oryza sativa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Oryza sativa |
General Information | Comment | Organism |
---|---|---|
additional information | tyrosine motif and a di-leucine motif mutants phenotype under both Fe-sufficient and -deficient conditions, overview. Mutant m6-sGFP converts S-adenosyl methionine into nicotinamine in vitro, whereas mutant m7sGFP does not show NAS enzyme activity | Oryza sativa |
physiological function | graminaceous plants release mugineic acid family phytosiderophores (MAs) to acquire iron from the soil. Deoxymugineic acid secretion from rice roots fluctuates throughout the day, and vesicles accumulate in roots before mugineic acid family phytosiderophores secretion. These vesicles are involved in nicotinamine and 2'-deoxymugineic acid biosynthesis. A tyrosine motif and a di-leucine motif, which have been reported to be involved in cellular transport, are conserved in NAS proteins in plants. The localization of enzyme NAS to vesicles and the transport of these vesicles are crucial steps in nicotinamine synthesis, leading to deoxymugineic acid synthesis and secretion in rice. The tyrosine motif is involved in vesicle movement, whereas the di-leucine motif appears to be involved in vesicle localization and OsNAS2 activity, which are crucial for the proper function of OsNAS2 | Oryza sativa |