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Literature summary for 2.5.1.39 extracted from

  • Uchida, K.; Koizumi, N.; Kawaji, H.; Kawahara, K.; Aida, K.
    Solubilization of 4-hydroxybenzoate-polyprenyltransferase from cell membrane of Pseudomonas putida and its properties (1991), Agric. Biol. Chem., 55, 2299-2305.
No PubMed abstract available
Search for more literature for 2.5.1.39

Activating Compound

Activating Compound Comment Organism Structure
cardiolipin
-
 Pseudomonas putida
dipalmitoylphosphatidylcholine
-
 Pseudomonas putida
phosphatidylethanolamine
-
 Pseudomonas putida
phosphatidylglycerol
-
 Pseudomonas putida
Phospholipid strongly activated by phospholipid extracted from the bacterial cell, phosphatidylethanolamine is most effective Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
2,4-dinitrophenol 16% inhibition at 1 mM Pseudomonas putida
Ag+ complete inhibition at 0.1 mM Pseudomonas putida
Ca2+ 46% inhibition at 1 mM Pseudomonas putida
Cd2+ complete inhibition at 0.1 mM Pseudomonas putida
Co2+ 28% inhibition at 0.1 mM Pseudomonas putida
Cu2+ complete inhibition at 0.1 mM Pseudomonas putida
Fe2+ 68% inhibition at 0.1 mM Pseudomonas putida
Hg2+ complete inhibition at 0.1 mM Pseudomonas putida
Mn2+ 68% inhibition at 0.1 mM Pseudomonas putida
N-ethylmaleimide 67% inhibition at 1 mM Pseudomonas putida
p-chloromercuribenzoate 95% inhibition at 0.01 mM Pseudomonas putida
Zn2+ complete inhibition at 0.1 mM Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.054
-
 4-hydroxybenzoate pH 7, 37°C Pseudomonas putida
0.14
-
 nonaprenyl diphosphate pH 7, 37°C Pseudomonas putida

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Pseudomonas putida 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
 IAM 1219
-
Pseudomonas putida IAM 1219
-
 IAM 1219
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
solanesyl diphosphate + 4-hydroxybenzoate i.e. nonaprenyl diphosphate Pseudomonas putida diphosphate + 3-solanesyl-4-hydroxybenzoate
-
 ?
solanesyl diphosphate + 4-hydroxybenzoate i.e. nonaprenyl diphosphate Pseudomonas putida IAM 1219 diphosphate + 3-solanesyl-4-hydroxybenzoate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.2
-
 Pseudomonas putida