Literature summary for 2.5.1.32 extracted from

Neudert, U.; Martinez-Ferez, I.M.; Fraser, P.D.; Sandmann, G.
Expression of an active phytoene synthase from Erwinia uredovora and biochemical properties of the enzyme (1998), Biochim. Biophys. Acta, 1392, 51-58.

Activating Compound

Activating Compound	Comment	Organism	Structure
ATP	required	Pantoea ananatis

Cloned(Commentary)

Cloned (Comment)	Organism
overexpressed to about 20% of the total cellular protein in Escherichia coli. Formation of the active phytoene synthase has the effect of suppressing the growth of the expressing strain. Inhibition of growth arises from the depletion of the substrate geranylgeranyl diphosphate which, in Escherichia coli, is necesssary for the synthesis of essential propyldiphosphate derivatives. In order to overcome the poor growth characteristics of the phytoene expressing strain, the enzyme level is increased by co-expressing the isoprenoid biosynthetic genes crtE and idi, encoding Erwinia geranylgeranyl diphosphate synthase and Rhodobacter isopentenyl diphosphate isomerase, respectively	Pantoea ananatis

Cloned (Comment)

Organism

overexpressed to about 20% of the total cellular protein in Escherichia coli. Formation of the active phytoene synthase has the effect of suppressing the growth of the expressing strain. Inhibition of growth arises from the depletion of the substrate geranylgeranyl diphosphate which, in Escherichia coli, is necesssary for the synthesis of essential propyldiphosphate derivatives. In order to overcome the poor growth characteristics of the phytoene expressing strain, the enzyme level is increased by co-expressing the isoprenoid biosynthetic genes crtE and idi, encoding Erwinia geranylgeranyl diphosphate synthase and Rhodobacter isopentenyl diphosphate isomerase, respectively

Pantoea ananatis

Inhibitors

Inhibitors	Comment	Organism	Structure
phosphate	-	Pantoea ananatis
squalestatin	IC50: 0.015 mM	Pantoea ananatis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	Mn2+ or Mg2+ required	Pantoea ananatis
Mn2+	Mn2+ or Mg2+ required	Pantoea ananatis

Organism

Organism	UniProt	Comment	Textmining
Pantoea ananatis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pantoea ananatis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
geranylgeranyl diphosphate + geranylgeranyl diphosphate	the same enzyme catalyzes two consecutive reactions: 1. the synthesis of prephytoene diphosphate and 2. the synthesis of phytoene	Pantoea ananatis	prephytoene diphosphate + diphosphate	-	?
prephytoene diphosphate	the same enzyme catalyzes two consecutive reactions: 1. the synthesis of prephytoene diphosphate and 2. the synthesis of phytoene	Pantoea ananatis	phytoene + diphosphate	-	?
prephytoene diphosphate	the phytoene formed in vitro is present in both a 15-cis and all-trans isomeric configuration	Pantoea ananatis	phytoene + diphosphate	-	?

Cofactor

Cofactor	Comment	Organism	Structure
ATP	required	Pantoea ananatis

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
0.015	-	IC50: 0.015 mM	Pantoea ananatis	squalestatin